Identification of MicroRNA 395a in 24-Epibrassinolide-Regulated Root Growth of Arabidopsis thaliana Using MicroRNA Arrays
by
Li-Ling Lin 1, Chia-Chi Wu 2, Hsuan-Cheng Huang 3,*, Huai-Ju Chen 4, Hsu-Liang Hsieh 4,* and Hsueh-Fen Juan 1,2,5,*
Li-Ling Lin 1, Chia-Chi Wu 2, Hsuan-Cheng Huang 3,*, Huai-Ju Chen 4, Hsu-Liang Hsieh 4,* and Hsueh-Fen Juan 1,2,5,*
1
Department of Life Science, National Taiwan University, Taipei 106, Taiwan
2
Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan
3
Institute of Biomedical Informatics, Center for Systems and Synthetic Biology, National Yang-Ming University, Taipei 112, Taiwan
4
Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan
5
Graduate Institute of Biomedical Electronic and Bioinformatics, National Taiwan University, Taipei 106, Taiwan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(7), 14270-14286; https://doi.org/10.3390/ijms140714270
Received: 20 May 2013 / Revised: 18 June 2013 / Accepted: 28 June 2013 / Published: 9 July 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs 2013)
Brassinosteroids (BRs) are endogenous plant hormones and are essential for normal plant growth and development. MicroRNAs (miRNAs) of Arabidopsis thaliana are involved in mediating cell proliferation in leaves, stress tolerance, and root development. The specifics of BR mechanisms involving miRNAs are unknown. Using customized miRNA array analysis, we identified miRNAs from A. thaliana ecotype Columbia (Col-0) regulated by 24-epibrassinolide (EBR, a highly active BR). We found that miR395a was significantly up-regulated by EBR treatment and validated its expression under these conditions. miR395a was over expressed in leaf veins and root tissues in EBR-treated miR395a promoter::GUS plants. We integrated bioinformatics methods and publicly available DNA microarray data to predict potential targets of miR395a. GUN5—a multifunctional protein involved in plant metabolic functions such as chlorophyll synthesis and the abscisic acid (ABA) pathway—was identified as a possible target. ABI4 and ABI5, both genes positively regulated by ABA, were down-regulated by EBR treatment. In summary, our results suggest that EBR regulates seedling development and root growth of A. thaliana through miR395a by suppressing GUN5 expression and its downstream signal transduction.
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Keywords:
brassinosteroids; miR395a; root growth; Arabidopsis thaliana; microRNA array
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This is an open access article distributed under the Creative Commons Attribution License
MDPI and ACS Style
Lin, L.-L.; Wu, C.-C.; Huang, H.-C.; Chen, H.-J.; Hsieh, H.-L.; Juan, H.-F. Identification of MicroRNA 395a in 24-Epibrassinolide-Regulated Root Growth of Arabidopsis thaliana Using MicroRNA Arrays. Int. J. Mol. Sci. 2013, 14, 14270-14286.
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