Next Article in Journal
Construction of a Full-Length Enriched cDNA Library and Preliminary Analysis of Expressed Sequence Tags from Bengal Tiger Panthera tigris tigris
Next Article in Special Issue
Human Chorionic Gonadotropin Beta Subunit Genes CGB1 and CGB2 are Transcriptionally Active in Ovarian Cancer
Previous Article in Journal
Current Status of Biomarkers for Prostate Cancer
Previous Article in Special Issue
Chemoresistance Is Associated with MUC1 and Lewis y Antigen Expression in Ovarian Epithelial Cancers
Open AccessArticle

Ubiquitin-Conjugating Enzyme 9 Promotes Epithelial Ovarian Cancer Cell Proliferation in Vitro

Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(6), 11061-11071; https://doi.org/10.3390/ijms140611061
Received: 23 April 2013 / Revised: 11 May 2013 / Accepted: 14 May 2013 / Published: 24 May 2013
(This article belongs to the Special Issue Genes and Pathways in the Pathogenesis of Ovarian Cancer)
Epithelial ovarian cancer (EOC) is one of the leading causes of cancer deaths in women worldwide. Ubiquitin-conjugating enzyme 9 (Ubc9), the sole conjugating enzyme for sumoylation, regulates protein function and plays an important role in sumoylation-mediated cellular pathways. Although sumoylation plays a key role in DNA repair and tumorgenesis, whether Ubc9 is involved in EOC progression remains unknown. In the present study, we constructed Ubc-9 expressed recombined plasmid pEGFP-N1-Ubc9. The mRNA levels of Ubc9 were confirmed in human ovarian cell lines before and after transfection with pEGFP-N1-Ubc9 or small interfering RNA (siRNA) targeted Ubc9 by real-time polymerase chain reaction (PCR). The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to observe the effect of Ubc9 on cell proliferation. The protein levels of Ubc9, and proliferation-related signals Akt and physphorylated Akt were determined by Western blot. Our results showed that proliferation of EOC cells increased significantly in Ubc9 overexpressing cells, but decreased in Ubc9 knockdown cells. The physphorylation of Akt showed similar trends. In addition, the inhibitor of PI3K/Akt signaling pathway, LY294002, dramatically inhibited the growth of Ubc9 overexpressing cells. Therefore, Ubc9 gene plays an important role in cell proliferation in EOC through PI3K/Akt signaling pathway. View Full-Text
Keywords: epithelial ovarian cancer (EOC); ubiquitin-conjugating enzyme 9 (Ubc9); proliferation; Akt epithelial ovarian cancer (EOC); ubiquitin-conjugating enzyme 9 (Ubc9); proliferation; Akt
MDPI and ACS Style

Dong, M.; Pang, X.; Xu, Y.; Wen, F.; Zhang, Y. Ubiquitin-Conjugating Enzyme 9 Promotes Epithelial Ovarian Cancer Cell Proliferation in Vitro. Int. J. Mol. Sci. 2013, 14, 11061-11071.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop