Next Article in Journal
Intercellular Signaling Pathway among Endothelia, Astrocytes and Neurons in Excitatory Neuronal Damage
Next Article in Special Issue
Detection of Quantitative Trait Loci (QTLs) for Resistances to Small Brown Planthopper and Rice Stripe Virus in Rice Using Recombinant Inbred Lines
Previous Article in Journal
Development of a Preclinical Therapeutic Model of Human Brain Metastasis with Chemoradiotherapy
Previous Article in Special Issue
Endoplasmic Reticulum (ER) Stress Response and Its Physiological Roles in Plants
Open AccessArticle

Two Common Bean Genotypes with Contrasting Response to Phosphorus Deficiency Show Variations in the microRNA 399-Mediated PvPHO2 Regulation within the PvPHR1 Signaling Pathway

1
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México (UNAM), Av. Universidad 1001, Cuernavaca 62210, Morelos, Mexico
2
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, UNAM, Av. Universidad 2001, Cuernavaca 62210, Morelos, Mexico
3
Institut National de la Recherche Agronomique, UMR Eco&Sols-Ecologie Fonctionnelle & Biogéochimie des Sols & Agroécosystèmes, 2 Place Viala, Montpellier F34060, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(4), 8328-8344; https://doi.org/10.3390/ijms14048328
Received: 20 February 2013 / Revised: 2 April 2013 / Accepted: 2 April 2013 / Published: 16 April 2013
(This article belongs to the Special Issue Abiotic and Biotic Stress Tolerance Mechanisms in Plants)
Crop production of the important legume, the common bean (Phaseolus vulgaris), is often limited by low phosphorus (P) in the soil. The genotypes, BAT477 and DOR364, of the common bean have contrasting responses to P starvation. Plants from the BAT477 P deficiency tolerant genotype showed higher phosphate content and root biomass as compared to the DOR364 plants under P starvation. The PvPHR1 transcription factor-signaling pathway plays an essential role in the response to P starvation. PvPHO2, a negative regulator of this pathway, encodes an ubiquitin E2 conjugase that promotes degradation of P-responsive proteins and is the target gene of PvmiR399. PvPHO2 is downregulated in BAT477 plants under P deficiency, while such a response is not observed in P-starved DOR364 plants. Five putative PvmiR399 binding sites were identified in the 5' UTR region in both genotypes. While four sites showed an identical DNA sequence, the fifth (binding site of PvPHO2 one) showed three base changes and higher complementarity scores in DOR364 as compared to BAT477. Modified 5'RACE experiments indicated that PvmiR399 binding and/or processing was affected in DOR364 P-starved plants. We propose that a less efficient cleavage of the PvPHO2 mRNA directed by PvmiR399 would result in a higher PvPHO2-mediated degradation of P-responsive proteins in the DOR364 genotype with decreased P deficiency tolerance. View Full-Text
Keywords: Phaseolus vulgaris; common bean; phosphorus deficiency; post-transcriptional regulation by microRNAs; PvPHR1/PvmiR399 signaling pathway Phaseolus vulgaris; common bean; phosphorus deficiency; post-transcriptional regulation by microRNAs; PvPHR1/PvmiR399 signaling pathway
MDPI and ACS Style

Ramírez, M.; Flores-Pacheco, G.; Reyes, J.L.; Luz Alvarez, A.; Drevon, J.J.; Girard, L.; Hernández, G. Two Common Bean Genotypes with Contrasting Response to Phosphorus Deficiency Show Variations in the microRNA 399-Mediated PvPHO2 Regulation within the PvPHR1 Signaling Pathway. Int. J. Mol. Sci. 2013, 14, 8328-8344.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop