Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs
Abstract
:1. Introduction
2. Results
2.1. miR-155 Is Upregulated in PBMCs of Active RA Patients
2.2. miR-155 Upregulation Correlates with Increased Production of TNF-α and IL-1β in RA Patients
2.3. miR-155 Promotes Production of TNF-α and IL-1β in Human Macrophage Cells
2.4. miR-155 Inhibitor Reduces the Production of Proinflammatory Cytokines Induced by LPS
2.5. miR-155 Targets SOCS1 Gene and Suppresses Its Expression
3. Discussion
4. Experimental Section
4.1. Subjects
4.2. Assay for TNF-α and IL-1β
4.3. Isolation, Culturing and Treatment of PBMCs
4.4. RNA Extraction and Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR)
4.5. Protein Isolation and Western Blot Analysis
4.6. Luciferase Activity Assay
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Index | RA (n = 45) | OA (n = 32) | Controls (n = 25) |
---|---|---|---|
Gender | |||
M | 13 (28.89%) | 1 (3.13%) | 2 (8.00%) |
F | 32 (71.11%) | 31 (96.88%) | 23 (92.00%) |
Age (years) | 42 (21–58) | 55 (44–62) | 39 (22–54) |
Duration of disease (years) | 7 (0.2–22) | 7.5 (3.5–12.5) | — |
DAS 28 | 5.7 (2.5–8.13) | — | — |
HAQ score | 44.6 (0.0–100.0) | — | — |
Therapy | |||
Conventional | 40 (86.96%) | ||
Anti-TNF-α | 6 (13.04%) | ||
ESR (mm/h) a | 36 (5–107) | 11 (7–17) | 9 (6–12) |
Anti-CCP (U/mL) a | 105 (41–830) | 9 (7.2–16.4) | 7.1 (5.1–14.3) |
TNF-α (pg/mL) ab | 25.20 (12–41.8) | 12.83 (8.4–16.9) | 9.92 (7.4–15.6) |
IL-1 (pg/mL) ab | 36.27 (15.42–59.17) | 22.45 (16.8–30) | 17.56 (13.2–23.4) |
Variables | Correlation | p-value |
---|---|---|
Age | −0.152 | 0.36 |
Disease duration | −0.478 | 0.098 |
Duration of morning stiffness | 0.316 | 0.064 |
DAS 28 | 0.372 | 0.009 a |
HAQ score | −0.015 | 0.76 |
RF | 0.125 | 0.48 |
ESR | 0.371 | 0.002 a |
Anti-CCP | 0.282 | 0.18 |
TNF-α | 0.369 | <0.0001 a |
IL-1β | 0.246 | 0.0005 a |
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Li, X.; Tian, F.; Wang, F. Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs. Int. J. Mol. Sci. 2013, 14, 23910-23921. https://doi.org/10.3390/ijms141223910
Li X, Tian F, Wang F. Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs. International Journal of Molecular Sciences. 2013; 14(12):23910-23921. https://doi.org/10.3390/ijms141223910
Chicago/Turabian StyleLi, Xiaochuan, Feng Tian, and Fei Wang. 2013. "Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs" International Journal of Molecular Sciences 14, no. 12: 23910-23921. https://doi.org/10.3390/ijms141223910