Next Article in Journal
Synthesis of 2-Acyloxycyclohexylsulfonamides and Evaluation on Their Fungicidal Activity
Next Article in Special Issue
Self/Co-Assembling Peptide, EAR8-II, as a Potential Carrier for a Hydrophobic Anticancer Drug Pirarubicin (THP)—Characterization and in-Vitro Delivery
Previous Article in Journal
A Review of Microsatellite Markers and Their Applications in Rice Breeding Programs to Improve Blast Disease Resistance
Previous Article in Special Issue
The Anti-Fasciolasis Properties of Silver Nanoparticles Produced by Trichoderma harzianum and Their Improvement of the Anti-Fasciolasis Drug Triclabendazole
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2013, 14(11), 22529-22543;

Size-Dependent Cytotoxicity of Nanocarbon Blacks

1,* and 2,*
School of Marine Sciences, Ningbo University, Ningbo 315211, China
Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
University of Chinese Academy of Sciences, Beijing 100049, China
Key Laboratory of Organofluorine Chemistry and Laboratory of Polymer Materials, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 22 September 2013 / Revised: 25 October 2013 / Accepted: 28 October 2013 / Published: 14 November 2013
(This article belongs to the Special Issue Bioactive Nanoparticles 2013)
Full-Text   |   PDF [1584 KB, uploaded 19 June 2014]   |  


In this study, we investigated the toxic effects of nanocarbon blacks (NCBs) with different sizes to mouse macrophage RAW264.7 cells. MTT and fluorescence-based LIVE assays demonstrated that NCBs uptake caused a size and dose-dependent growth inhibition to the cells. Optical microscopy observations and 99mTc radionuclide labeling techniques were used to investigate the cellular uptake of NCBs with different sizes qualitatively and quantitatively, respectively. Results showed that the cellular uptake amounts of NCBs increased with their increasing size. Large quantities of internal NCBs induced oxidative stress and nuclear damage in cells; these effects may be the critical factors involved in the cytotoxicity of NCBs. The implications associated with these findings are discussed. View Full-Text
Keywords: nanocarbon blacks (NCBs); size; cytotoxicity; cellular uptake; cytotoxic mechanism nanocarbon blacks (NCBs); size; cytotoxicity; cellular uptake; cytotoxic mechanism

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Kong, H.; Zhang, Y.; Li, Y.; Cui, Z.; Xia, K.; Sun, Y.; Zhao, Q.; Zhu, Y. Size-Dependent Cytotoxicity of Nanocarbon Blacks. Int. J. Mol. Sci. 2013, 14, 22529-22543.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top