Isolation and Molecular Characterization of Thirteen R2R3-MYB Transcription Factors from Epimedium sagittatum
Abstract
:1. Introduction
2. Results
2.1. Isolation and Sequence Analysis of 13 R2R3-MYB Genes from Epimedium
2.2. Phylogenetic Relationships and Genomic Structure Analysis of Epimedium R2R3-MYB Genes
2.3. Expression Patterns of Epimedium R2R3-MYB Genes in Various Tissues
3. Discussion
4. Experimental Section
4.1. Plant Materials
4.2. DNA and RNA Extraction
4.3. Reverse Transcription and Isolation of R2R3-MYB cDNA Clones
4.4. Sequence and Phylogenetic Analysis
4.5. Quantitative RT-PCR
4.6. Total Anthocyanin Content Determination
5. Conclusions
Supplementary Information
ijms-14-00594-s001.pdfSupplementary Information
ijms-14-00594-s002.pdfAcknowledgments
- Conflict of InterestThe authors declare no conflict of interest.
References
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Huang, W.; Sun, W.; Lv, H.; Xiao, G.; Zeng, S.; Wang, Y. Isolation and Molecular Characterization of Thirteen R2R3-MYB Transcription Factors from Epimedium sagittatum. Int. J. Mol. Sci. 2013, 14, 594-610. https://doi.org/10.3390/ijms14010594
Huang W, Sun W, Lv H, Xiao G, Zeng S, Wang Y. Isolation and Molecular Characterization of Thirteen R2R3-MYB Transcription Factors from Epimedium sagittatum. International Journal of Molecular Sciences. 2013; 14(1):594-610. https://doi.org/10.3390/ijms14010594
Chicago/Turabian StyleHuang, Wenjun, Wei Sun, Haiyan Lv, Gong Xiao, Shaohua Zeng, and Ying Wang. 2013. "Isolation and Molecular Characterization of Thirteen R2R3-MYB Transcription Factors from Epimedium sagittatum" International Journal of Molecular Sciences 14, no. 1: 594-610. https://doi.org/10.3390/ijms14010594