Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation

Lin, Li-Ling; Huang, Hsuan-Cheng; Ogihara, Satoshi; Wang, Jin-Town; Wu, Meng-Chuan; McNeil, Paul L.; Chen, Chiung-Nien; Juan, Hsueh-Fen

doi:10.3390/ijms130810176

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Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation

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Institute of Molecular and Cellular Biology and Department of Life Science, National Taiwan University, Taipei 106, Taiwan

Institute of Biomedical Informatics, Center for Systems and Synthetic Biology, National Yang-Ming University, Taipei 112, Taiwan

Biological Science, Graduate School of Science, Osaka University, Osaka 560-0043, Japan

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Department of Microbiology, National Taiwan University College of Medicine, Taipei 100, Taiwan

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Department of Cellular Biology and Anatomy and Institute of Molecular Medicine and Genetics, Georgia Health Science University, 1120 15th St., Augusta, GA 30912, USA

⁶

Angiogenesis Research Center, National Taiwan University, Taipei 106, Taiwan

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Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2012, 13(8), 10176-10192; https://doi.org/10.3390/ijms130810176

Received: 5 June 2012 / Revised: 3 August 2012 / Accepted: 7 August 2012 / Published: 15 August 2012

(This article belongs to the Special Issue Advances in Molecular Oncology (special issue))

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Abstract

Helicobacter pylori (H. pylori), the human stomach pathogen, lives on the inner surface of the stomach and causes chronic gastritis, peptic ulcer, and gastric cancer. Plasma membrane repair response is a matter of life and death for human cells against physical and biological damage. We here test the hypothesis that H. pylori also causes plasma membrane disruption injury, and that not only a membrane repair response but also a cell proliferation response are thereby activated. Vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA) have been considered to be major H. pylori virulence factors. Gastric cancer cells were infected with H. pylori wild type (vacA+/cagA+), single mutant (ΔvacA or ΔcagA) or double mutant (ΔvacA/ΔcagA) strains and plasma membrane disruption events and consequent activation of membrane repair components monitored. H. pylori disrupts the host cell plasma membrane, allowing localized dye and extracellular Ca²⁺ influx. Ca²⁺-triggered members of the annexin family, A1 and A4, translocate, in response to injury, to the plasma membrane, and cell surface expression of an exocytotic maker of repair, LAMP-2, increases. Additional forms of plasma membrane disruption, unrelated to H. pylori exposure, also promote host cell proliferation. We propose that H. pylori activation of a plasma membrane repair is pro-proliferative. This study might therefore provide new insight into potential mechanisms of H. pylori-induced gastric carcinogenesis. View Full-Text

Keywords: plasma membrane repair; Helicobacter pylori; cell proliferation; annexin A4

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Lin, L.-L.; Huang, H.-C.; Ogihara, S.; Wang, J.-T.; Wu, M.-C.; McNeil, P.L.; Chen, C.-N.; Juan, H.-F. Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation. Int. J. Mol. Sci. 2012, 13, 10176-10192. https://doi.org/10.3390/ijms130810176

AMA Style

Lin L-L, Huang H-C, Ogihara S, Wang J-T, Wu M-C, McNeil PL, Chen C-N, Juan H-F. Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation. International Journal of Molecular Sciences. 2012; 13(8):10176-10192. https://doi.org/10.3390/ijms130810176

Chicago/Turabian Style

Lin, Li-Ling, Hsuan-Cheng Huang, Satoshi Ogihara, Jin-Town Wang, Meng-Chuan Wu, Paul L. McNeil, Chiung-Nien Chen, and Hsueh-Fen Juan. 2012. "Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation" International Journal of Molecular Sciences 13, no. 8: 10176-10192. https://doi.org/10.3390/ijms130810176

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Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation

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