Next Article in Journal
3D Structure Elucidation of Thermostable L2 Lipase from Thermophilic Bacillus sp. L2
Previous Article in Journal
Effect of β,β-Dimethylacrylshikonin on Inhibition of Human Colorectal Cancer Cell Growth in Vitro and in Vivo
Article

Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus

1
College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China
2
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2012, 13(7), 9194-9206; https://doi.org/10.3390/ijms13079194
Received: 22 May 2012 / Revised: 4 July 2012 / Accepted: 16 July 2012 / Published: 23 July 2012
The mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from I. obliquus, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were measured. Their antioxidant properties against chemicals-induced reactive species (ROS) including 1,1'-Diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide anion radical, as well as their protective effects on H2O2-induced PC12 cell death were investigated. Results showed that I. obliquus polysaccharides can scavenge all ROS tested above in a dose-dependent manner. IOA and its product IOA-1 could rescue PC12 cell viability from 38.6% to 79.8% and 83.0% at a concentration of 20µg/mL. Similarly, IOW and its product IOW-1 at the same dose, can also increase cell viability to 84.9% and 88.6% respectively. The antioxidative activities of water-soluble and alkali-soluble polysaccharide constituents from I. obliquus might contribute to diverse medicinal and nutritional values of this mushroom. View Full-Text
Keywords: Inonotus obliquus; polysaccharide; antioxidant; reactive oxygen species Inonotus obliquus; polysaccharide; antioxidant; reactive oxygen species
Show Figures

MDPI and ACS Style

Mu, H.; Zhang, A.; Zhang, W.; Cui, G.; Wang, S.; Duan, J. Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus. Int. J. Mol. Sci. 2012, 13, 9194-9206. https://doi.org/10.3390/ijms13079194

AMA Style

Mu H, Zhang A, Zhang W, Cui G, Wang S, Duan J. Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus. International Journal of Molecular Sciences. 2012; 13(7):9194-9206. https://doi.org/10.3390/ijms13079194

Chicago/Turabian Style

Mu, Haibo, Amin Zhang, Wuxia Zhang, Guoting Cui, Shunchun Wang, and Jinyou Duan. 2012. "Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus" International Journal of Molecular Sciences 13, no. 7: 9194-9206. https://doi.org/10.3390/ijms13079194

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop