Comparison of Ultrasonic and CO2 Laser Pretreatment Methods on Enzyme Digestibility of Corn Stover
Abstract
:1. Introduction
2. Results and Discussion
2.1. Compositional Analysis of Raw Corn Stover Sample
2.2. Enzymatic Hydrolysis
2.3. Effect of Pretreatment on Sugar Content Hydrolysate
2.4. Analysis of the FT-IR Spectroscopy
2.5. Effect of Pretreatments on Morphology
2.6. Mechanisms of LAM Pretreatment
3. Experimental Section
3.1. Materials and Equipment
3.2. Composition of Corn Stover Biomass
3.3. Pretreatment of Corn Stover Samples
3.4. Enzymatic Hydrolysis of Pretreated Corn Stover
3.5. Hydrolysate Analysis
3.6. Spectral Analysis of Pretreated Samples
3.7. Surface Morphology Observation by SEM
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Sample | Xylose (mg/g biomass) | Glucose (mg/g biomass) | Cellobiose (mg/g biomass) |
---|---|---|---|
CO2 laser pretreated hydrolysate | 15.03 | 131.20 | 4.67 |
Ultrasonic pretreated hydrolysate | 20.61 | 101.13 | 7.07 |
Non-pretreatment hydrolysate | 5.33 | 15.00 | trace amount |
Wave Number(σ/cm−1) | Intensity of Absorption Band | Absorption Peak Assignment |
---|---|---|
3338 | steep | OH stretching in alcohol and phenol |
2921 | moderate | C–H symmetrical and asymmetrical stretching in –CH3 and –CH2– Organic acid COO– asymmetrical stretch |
1650–1630 | semi-steep | Lignin and aromatic ring conjugated C=O stretch |
1509–1515 | moderate | Lignin and other aromatic ring skeletal stretch –CH2– scissoring deformation in carbonhydrates and fatty compounds |
1462 | infirm | C–H deformations (asym. in –CH3 and –CH2–) in lignin and carbonhydrates |
1421 | steep | Aromatic skeletal vibrations combined with C–H in-plane deformations |
1325 | moderate | C–H vibration in cellulose and C1–O vibration in syringyl derivatives |
1265 | moderate | Aromatic skeletal vibrations, guaiacyl, C=O stretch |
1160 | faint | C–O–C vibration in cellulose and hemicellulose |
1117–1124 | infirm | C–H aromatic ring, syringyl C–O stretch in cellulose and hemicellulose |
1049 | moderate | Si–O stretch in amorphous SiO2 |
898 | faint | C–H deformation in cellulose and saccharide |
666 | faint | Single-plane vibration of substituted aromatics |
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Tian, S.-Q.; Wang, Z.-Y.; Fan, Z.-L.; Zuo, L.-L. Comparison of Ultrasonic and CO2 Laser Pretreatment Methods on Enzyme Digestibility of Corn Stover. Int. J. Mol. Sci. 2012, 13, 4141-4152. https://doi.org/10.3390/ijms13044141
Tian S-Q, Wang Z-Y, Fan Z-L, Zuo L-L. Comparison of Ultrasonic and CO2 Laser Pretreatment Methods on Enzyme Digestibility of Corn Stover. International Journal of Molecular Sciences. 2012; 13(4):4141-4152. https://doi.org/10.3390/ijms13044141
Chicago/Turabian StyleTian, Shuang-Qi, Zhen-Yu Wang, Zi-Luan Fan, and Li-Li Zuo. 2012. "Comparison of Ultrasonic and CO2 Laser Pretreatment Methods on Enzyme Digestibility of Corn Stover" International Journal of Molecular Sciences 13, no. 4: 4141-4152. https://doi.org/10.3390/ijms13044141