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Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study

Laboratory for Optics and Biosciences, Ecole Polytechnique/CNRS/INSERM, 91128 Palaiseau cedex, France
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2012, 13(2), 2239-2248;
Received: 5 January 2012 / Revised: 2 February 2012 / Accepted: 13 February 2012 / Published: 17 February 2012
(This article belongs to the Special Issue Applications of Circular Dichroism)
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Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we are able to measure precisely the unfolding relaxation time as well as the helical fraction evolution. We show that only CD at 220 nm is relevant to observe the unfolding of an alpha helix whereas no change is observed for CD at 204 nm. Conversely, both absorptions yield information on the dynamics of the process. View Full-Text
Keywords: circular dichroism; poly(glutamic acid); T-jump circular dichroism; poly(glutamic acid); T-jump
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Mendonça, L.; Hache, F. Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study. Int. J. Mol. Sci. 2012, 13, 2239-2248.

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