Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer
Abstract
:1. Introduction
2. Results and Discussion
2.1. Expression of Cav-1 and LC3B in GC
2.2. Clinical Significance and Prognostic Value of Fibroblastic Cav-1 and LC3B
2.3. The Correlation between Fibroblastic Cav-1 and LC3B
2.4. EBV Infection Affected Expression of Fibroblastic Cav-1 and LC3B
2.5. Discussion
3. Experimental Section
3.1. Patients and Tissue Samples
3.2. Tissue Microarray Construction
3.3. QDs-Based Immunofluorescence Histochemistry
3.4. QDs-Based Double Immunofluorescence Labeling
3.5. QDs-Based Fluorescence in situ Hybridization
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
- Conflict of InterestThe authors declare no conflict of interest.
References
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Features | n | Fibroblastic Cav-1 | p | n | Fibroblastic LC3B | p | ||
---|---|---|---|---|---|---|---|---|
Low (%) | High (%) | Negative (%) | Positive (%) | |||||
Age | 0.724 | 0.287 | ||||||
<58 | 55 | 28 (50.9) | 27 (40.1) | 50 | 39 (78.0) | 11 (22.0) | ||
≥58 | 63 | 30 (47.6) | 33 (52.4) | 51 | 35 (68.6) | 16 (31.4) | ||
Gender | 0.186 | 0.384 | ||||||
Male | 85 | 45 (52.9) | 40 (47.1) | 72 | 51 (70.8) | 21 (29.2) | ||
Female | 33 | 13 (39.4) | 20 (60.6) | 29 | 23 (79.3) | 6 (20.7) | ||
Depth of invasion # | 0.903 | 0.032 | ||||||
T1 + T2 | 36 | 18 (50.0) | 18 (50.0) | 32 | 19 (59.4) | 13 (40.6) | ||
T3 + T4 | 82 | 40 (48.8) | 42 (51.2) | 69 | 55 (79.7) | 14 (20.3) | ||
Lymph node status # | 0.474 | 0.992 | ||||||
N0 | 39 | 21 (53.8) | 18 (46.2) | 30 | 22 (73.3) | 8 (26.7) | ||
N1 + N2 | 79 | 37 (46.8) | 42 (53.2) | 71 | 52 (73.2) | 19 (26.8) | ||
TNM stage # | 0.725 | 0.914 | ||||||
0+Ia+Ib+II | 53 | 27 (50.9) | 26 (49.1) | 44 | 32 (72.7) | 12 (27.3) | ||
IIIa+ IIIb+ IV | 65 | 31 (47.7) | 34 (52.3) | 57 | 42 (73.7) | 15 (26.3) | ||
Grade of AC | 0.792 | 0.065 | ||||||
Well and moderately | 43 | 20 (46.5) | 23 (53.5) | 34 | 20 (58.8) | 14 (41.2) | ||
Poorly | 59 | 29 (49.2) | 30 (50.8) | 53 | 41 (77.4) | 12 (22.6) | ||
Lauren classification | 0.316 | 0.142 | ||||||
Intestinal-type | 65 | 28 (43.1) | 37 (56.9) | 51 | 31 (60.8) | 20 (39.2) | ||
Diffuse-type | 45 | 26 (57.8) | 19 (42.2) | 42 | 36 (85.7) | 6 (14.3) | ||
Mixed-type | 8 | 4 (50.0) | 4 (50.0) | 8 | 7 (87.5) | 1 (12.5) | ||
HER-2 | 0.967 | 0.628 | ||||||
Positive | 23 | 11 (47.8) | 12 (52.2) | 23 | 16 (69.6) | 7 (30.4) | ||
Negative | 89 | 43 (48.3) | 46 (51.7) | 75 | 56 (74.7) | 19 (25.3) | ||
Tumor cell type | 0.565 | 0.189 | ||||||
AC | 102 | 49 (48.0) | 53 (52.0) | 87 | 61 (70.1) | 26 (29.9) | ||
MAC | 11 | 7 (63.6) | 4 (36.4) | 10 | 9 (90.0) | 1 (10.0) | ||
UC | 5 | 2 (40.0) | 3 (60.0) | 4 | 4 (100.0) | 0 (0) | ||
Fibroblastic Cav-1 | 0.000 | |||||||
Low | 45 | 42 (93.3) | 3 (6.7) | |||||
High | 51 | 28 (54.9) | 23 (45.1) |
Factors | Univariate analysis | Multivariate analysis | ||
---|---|---|---|---|
p value | HR (95%CI) | p value | HR (95%CI) | |
Sex | ||||
Men vs. women | 0.732 | 1.098 (0.638, 1.890) | ||
Age | ||||
<56 vs. ≥56 | 0.199 | 0.728 (0.445, 1.193) | ||
TNM stage | ||||
I–II vs. III–IV | 0.003 | 0.543 (0.256, 0.803) | 0.001 | 4.344 (1.895, 9.958) |
T classification | ||||
T1-T2 vs. T3-T4 | 0.001 | 0.317 (0.137, 0.732) | 0.483 | |
Lymph node | ||||
No vs. Yes | 0.034 | 0.528 (0.275, 0.987) | 0.663 | |
Lauren classification | ||||
Intestinal-type vs. Diffuse-type | 0.882 | 0.965 (0.603, 1.543) | ||
Grade of AC | ||||
Well and moderately vs. poorly | 0.638 | 0.873 (0.493, 1.546) | ||
Fibroblastic Cav-1 | ||||
High vs. Low | 0.021 | 0.556 (0.332, 0.929) | 0.029 | 0.474 (0.242, 0.928) |
Fibroblastic LC3B | ||||
Positive vs. Negative | 0.041 | 0.498 (0.235, 1.055) | 0.180 | |
EBV infection | ||||
Yes vs. No | 0.347 | 0.725 (0.355, 1.481) |
Variables | Fibroblastic Cav-1 | Fibroblastic LC3B | |||||||
---|---|---|---|---|---|---|---|---|---|
Low (%) | High (%) | r | p | Negative (%) | Positive (%) | r | p | ||
EBV Infection | Negative | 48 (49.5) | 49 (50.0) | 0.092 | 0.877 | 60(74.1) | 21 (25.9) | 0.037 | 0.712 |
Positive | 10 (47.6) | 11 (52.4) | 14(70.0) | 6 (30.0) |
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He, Y.; Zhao, X.; Gao, J.; Fan, L.; Yang, G.; Cho, W.C.-s.; Chen, H. Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer. Int. J. Mol. Sci. 2012, 13, 13764-13780. https://doi.org/10.3390/ijms131113764
He Y, Zhao X, Gao J, Fan L, Yang G, Cho WC-s, Chen H. Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer. International Journal of Molecular Sciences. 2012; 13(11):13764-13780. https://doi.org/10.3390/ijms131113764
Chicago/Turabian StyleHe, Yuyu, Xianda Zhao, Jun Gao, Lifang Fan, Guifang Yang, William Chi-shing Cho, and Honglei Chen. 2012. "Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer" International Journal of Molecular Sciences 13, no. 11: 13764-13780. https://doi.org/10.3390/ijms131113764