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Open AccessArticle

Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer

1
Department of Pathology, School of Basic Medical Science, Wuhan University, Wuhan 430071, China
2
Department of Molecular Pathology, Wuhan Nano Tumor Diagnosis Engineering Research Center, Wuhan 430075, China
3
Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
4
Department of Clinical Oncology, Queen Elizabeth Hospital, 30 Gascoigne Road, Kowloon, Hong Kong
5
Department of Biochemistry, Rush University Medical Center, 1735 West Harrison Street, Chicago, IL 60612, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2012, 13(11), 13764-13780; https://doi.org/10.3390/ijms131113764
Received: 13 September 2012 / Revised: 10 October 2012 / Accepted: 16 October 2012 / Published: 24 October 2012
(This article belongs to the Special Issue Advances in Molecular Oncology (special issue))
Caveolin-1 (Cav-1) expression deficiency and autophagy in tumor stromal fibroblasts (hereafter fibroblasts) are involved in tumor proliferation and progression, particularly in breast and prostate cancer. The aim of this study was to detect the expression of fibroblastic Cav-1 and LC3B, markers of autophagy, in gastric cancer (GC) and to analyze their clinical significances. Furthermore, because Epstein-Barr virus (EBV)-associated GC (EBVaGC) is a unique subtype of GC; we compared the differential expression of fibroblastic Cav-1 and LC3B in EBVaGC and non-EBVaGC. Quantum dots (QDs)-based immunofluorescence histochemistry was used to examine the expression of fibroblastic Cav-1 and LC3B in 118 cases of GC with adequate stroma. QDs-based double immunofluorescence labeling was performed to detect the coexpression of Cav-1 and LC3B proteins. EBV-encoded small RNA was detected by QDs-based fluorescence in situ hybridization to identify EBVaGC. Multivariate analysis indicated that low fibroblastic Cav-1 level was an independent prognosticator (p = 0.029) that predicted poorer survival of GC patients. Positive fibroblastic LC3B was correlated with lower invasion (p = 0.032) and was positively associated with Cav-1 expression (r = 0.432, p < 0.001). EBV infection did not affect fibroblastic Cav-1 and LC3B expression. In conclusion, positive fibroblastic LC3B correlates with lower invasion, and low expression of fibroblastic Cav-1 is a novel predictor of poor GC prognosis. View Full-Text
Keywords: autophagy; cancer-associated fibroblast; caveolin-1; gastric cancer; light chain 3B; quantum dots; tumor microenvironment autophagy; cancer-associated fibroblast; caveolin-1; gastric cancer; light chain 3B; quantum dots; tumor microenvironment
MDPI and ACS Style

He, Y.; Zhao, X.; Gao, J.; Fan, L.; Yang, G.; Cho, W.C.-S.; Chen, H. Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer. Int. J. Mol. Sci. 2012, 13, 13764-13780.

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