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Int. J. Mol. Sci. 2012, 13(10), 12890-12910;

Fluorescence Lifetime Correlation Spectroscopy (FLCS): Concepts, Applications and Outlook

J. Heyrovský Institute of Physical Chemistry of ASCR, v.v.i, Dolejškova 3, 18223 Prague 8, Czech Republic
Czech Technical University in Prague, Faculty of Biomedical Engineering, Sítná 3105, 272 01 Kladno, Czech Republic
Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia
Author to whom correspondence should be addressed.
Received: 15 August 2012 / Revised: 14 September 2012 / Accepted: 19 September 2012 / Published: 9 October 2012
(This article belongs to the Special Issue Advances in Single Molecule Spectroscopy)
Full-Text   |   PDF [400 KB, uploaded 19 June 2014]


Fluorescence Lifetime Correlation Spectroscopy (FLCS) is a variant of fluorescence correlation spectroscopy (FCS), which uses differences in fluorescence intensity decays to separate contributions of different fluorophore populations to FCS signal. Besides which, FLCS is a powerful tool to improve quality of FCS data by removing noise and distortion caused by scattered excitation light, detector thermal noise and detector afterpulsing. We are providing an overview of, to our knowledge, all published applications of FLCS. Although these are not numerous so far, they illustrate possibilities for the technique and the research topics in which FLCS has the potential to become widespread. Furthermore, we are addressing some questions which may be asked by a beginner user of FLCS. The last part of the text reviews other techniques closely related to FLCS. The generalization of the idea of FLCS paves the way for further promising application of the principle of statistical filtering of signals. Specifically, the idea of fluorescence spectral correlation spectroscopy is here outlined. View Full-Text
Keywords: fluorescence correlation spectroscopy (FCS); time correlated single photon counting (TCSPC); fluorescence cross-correlation spectroscopy (FCCS) fluorescence correlation spectroscopy (FCS); time correlated single photon counting (TCSPC); fluorescence cross-correlation spectroscopy (FCCS)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Kapusta, P.; Macháň, R.; Benda, A.; Hof, M. Fluorescence Lifetime Correlation Spectroscopy (FLCS): Concepts, Applications and Outlook. Int. J. Mol. Sci. 2012, 13, 12890-12910.

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