Chromatographic Fingerprint and the Simultaneous Determination of Five Bioactive Components of Geranium carolinianum L. Water Extract by High Performance Liquid Chromatography
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of Sample Preparation
2.2. Optimization of HPLC Conditions
2.3. Validation of Fingerprint Analysis
2.4. Validation of Quantitative Analysis
2.5. HPLC Fingerprint Analysis
2.6. HPLC Quantitative Analysis
3. Experimental Section
3.1. Materials and Reagents
3.2. Instrumentation and Chromatographic Conditions
3.3. Sample Preparation
3.4. Analysis of Chromatographic Fingerprints
4. Conclusions
Acknowledgments
- Conflicts of InterestThe authors declare no conflicts of interest.
References
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Standard Substance | Calibration Equation y = ax + b (a) | R (b) | Linear Range (μg/mL) | LOD (μg/mL) | LOQ (μg/mL) |
---|---|---|---|---|---|
Gallic acid | y = 2619.1x – 15.114 | 0.9998 | 7.00–140.00 | 0.01 | 0.035 |
Corilagin | y = 1338.5x – 66.433 | 1.0000 | 65.00–780.00 | 0.055 | 0.22 |
Methyl brevifolincarboxylate | y = 4955.2x + 65.426 | 0.9993 | 5.00–40.00 | 0.35 | 1.00 |
Ellagic acid | y = 2467.0x – 3.9683 | 0.9998 | 10.00–100.00 | 0.10 | 0.25 |
Rutin | y = 961.98x – 56.558 | 0.9991 | 10.00–160.00 | 0.70 | 2.00 |
Standard Substance | Precision RSD (%) (n = 6) | Repeatability RSD (%) (n = 5) | Stability RSD (%) (n = 5) | Recovery (%) (a) (n = 9) Mean RSD (%) | |
---|---|---|---|---|---|
Gallic acid | 1.88 | 4.44 | 0.82 | 97.2 | 3.19 |
Corilagin | 3.25 | 0.63 | 0.54 | 99.5 | 3.77 |
Methyl brevifolincarboxylate | 4.99 | 4.36 | 1.74 | 98.0 | 4.50 |
Ellagic acid | 2.80 | 4.39 | 3.28 | 100.3 | 4.42 |
Rutin | 2.36 | 1.67 | 4.35 | 101.7 | 4.21 |
Sample | Species | Habit | Collection time | Similarities |
---|---|---|---|---|
S1 | G. carolinianum L. | Suburb of Nanjing | April, 2011 | 0.993 |
S2 | G. carolinianum L. | Suburb of Nanjing | April, 2011 | 0.997 |
S3 | G. carolinianum L. | Suburb of Nanjing | May, 2011 | 0.998 |
S4 | G. carolinianum L. | Suburb of Nanjing | May, 2011 | 0.993 |
S5 | G. carolinianum L. | Suburb of Nanjing | May, 2011 | 0.999 |
S6 | G. carolinianum L. | Suburb of Nanjing | May, 2011 | 0.997 |
S7 | G. carolinianum L. | Suburb of Nanjing | May, 2011 | 0.997 |
S8 | G. carolinianum L. | Suburb of Nanjing | May, 2011 | 0.997 |
S9 | G. carolinianum L. | Suburb of Nanjing | May, 2011 | 0.996 |
S10 | G. carolinianum L. | Suburb of Nanjing | May, 2011 | 0.995 |
Sample | 2 (a) | 10 (b) | 11 (c) | 15 (d) | 16 (e) | Total |
---|---|---|---|---|---|---|
S1 | 7.48 | 41.37 | 1.80 | 3.16 | 4.29 | 58.10 |
S2 | 9.31 | 49.92 | 1.79 | 5.15 | 5.67 | 71.84 |
S3 | 7.51 | 41.53 | 1.81 | 3.17 | 4.30 | 58.32 |
S4 | 7.99 | 32.31 | 0.96 | 2.83 | 2.03 | 46.12 |
S5 | 8.45 | 43.74 | 1.48 | 5.16 | 3.00 | 61.83 |
S6 | 7.02 | 34.48 | 1.08 | 3.64 | 2.37 | 48.59 |
S7 | 6.74 | 30.85 | 0.99 | 3.51 | 2.19 | 44.28 |
S8 | 6.79 | 36.19 | 1.37 | 3.47 | 2.54 | 50.36 |
S9 | 9.15 | 43.15 | 1.49 | 5.14 | 3.27 | 62.20 |
S10 | 7.71 | 37.42 | 1.58 | 4.55 | 2.59 | 53.85 |
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Wu, Q.-Y.; Zhou, Y.; Jin, X.; Guan, Y.; Xu, M.; Liu, L.-F. Chromatographic Fingerprint and the Simultaneous Determination of Five Bioactive Components of Geranium carolinianum L. Water Extract by High Performance Liquid Chromatography. Int. J. Mol. Sci. 2011, 12, 8740-8749. https://doi.org/10.3390/ijms12128740
Wu Q-Y, Zhou Y, Jin X, Guan Y, Xu M, Liu L-F. Chromatographic Fingerprint and the Simultaneous Determination of Five Bioactive Components of Geranium carolinianum L. Water Extract by High Performance Liquid Chromatography. International Journal of Molecular Sciences. 2011; 12(12):8740-8749. https://doi.org/10.3390/ijms12128740
Chicago/Turabian StyleWu, Qiu-Yue, Yang Zhou, Xin Jin, Yue Guan, Min Xu, and Li-Fang Liu. 2011. "Chromatographic Fingerprint and the Simultaneous Determination of Five Bioactive Components of Geranium carolinianum L. Water Extract by High Performance Liquid Chromatography" International Journal of Molecular Sciences 12, no. 12: 8740-8749. https://doi.org/10.3390/ijms12128740