Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications
Abstract
:1. Introduction
2. Results and Discussion
2.1. Morphology of CS/SF Blend Nanofibers
2.2. Crosslinking of Fibers
2.3. Mechanical Properties
2.4. Evaluation of Antibacterial Activity in Vitro
2.5. Cell Morphology and Proliferation
3. Experimental Section
3.1. Materials
3.2. Preparation of Regenerated SF
3.3. Electrospinning
3.4. Glutaraldehyde Vapor Crosslinking of Nanofibers
3.5. Scanning Electron Microscopy Analysis
3.6. Mechanical Properties
3.7. Antibacterial Assessment
3.8. Hematoxylin and Eosin (H&E) Staining and MTT Analysis
3.9. Statistical Analysis
4. Conclusions
Acknowledgements
References
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Crosslinked CS/SF (wt/wt) | 0:10 | 2:8 | 5:5 | 8:2 |
---|---|---|---|---|
Tensile stress (MPa) | 10.3 ± 0.24 | 1.2 ± 0.13 | 1.1 ± 0.22 | 1.0 ± 0.21 |
Ultimate strain (%) | 2.8 ± 0.22 | 3.8 ± 0.21 | 2.5 ± 0.25 | 1.3 ± 0.20 |
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Cai, Z.-x.; Mo, X.-m.; Zhang, K.-h.; Fan, L.-p.; Yin, A.-l.; He, C.-l.; Wang, H.-s. Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications. Int. J. Mol. Sci. 2010, 11, 3529-3539. https://doi.org/10.3390/ijms11093529
Cai Z-x, Mo X-m, Zhang K-h, Fan L-p, Yin A-l, He C-l, Wang H-s. Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications. International Journal of Molecular Sciences. 2010; 11(9):3529-3539. https://doi.org/10.3390/ijms11093529
Chicago/Turabian StyleCai, Zeng-xiao, Xiu-mei Mo, Kui-hua Zhang, Lin-peng Fan, An-lin Yin, Chuang-long He, and Hong-sheng Wang. 2010. "Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications" International Journal of Molecular Sciences 11, no. 9: 3529-3539. https://doi.org/10.3390/ijms11093529