The Mechanism of (R,R) ZX-5 on Increasing NO Release
Abstract
:1. Introduction
2. Materials and Methods
2.1. The Structure of (R,R) ZX-5
2.2. Cell Culture
2.3. Measurement of NO Release
2.4. Reverse-Transcriptase Polymerase Chain Reaction Analysis of eNOS and iNOS Expression
2.5. Western Blot Analysis
2.6. Endothelial NO Synthase (eNOS) Activity Assay
2.7. Statistical Analysis
3. Results
3.1. (R,R) ZX-5 Treatment Increases NO Production in HUVECs
3.2. (R,R) ZX-5 Up-Regulates iNOS mRNA in a Dose-Dependent Manner
3.3. (R,R) ZX-5 Treatment Leads to Increased iNOS and eNOS Protein Expression
3.4. (R,R) ZX-5 Treatment Inhibits ERK and AKT Expression
3.5. (R,R) ZX-5 Enhances eNOS Activity in HUVECs
4. Discussion
Acknowledgments
References
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Xu, H.-M.; Wei, J.; Pan, L.; Lin, H.; Wang, W.; Zhang, Y.; Shen, Z. The Mechanism of (R,R) ZX-5 on Increasing NO Release. Int. J. Mol. Sci. 2010, 11, 3323-3333. https://doi.org/10.3390/ijms11093323
Xu H-M, Wei J, Pan L, Lin H, Wang W, Zhang Y, Shen Z. The Mechanism of (R,R) ZX-5 on Increasing NO Release. International Journal of Molecular Sciences. 2010; 11(9):3323-3333. https://doi.org/10.3390/ijms11093323
Chicago/Turabian StyleXu, Han-Mei, Jin Wei, Li Pan, Hongying Lin, Weiqiang Wang, Yihua Zhang, and Zilong Shen. 2010. "The Mechanism of (R,R) ZX-5 on Increasing NO Release" International Journal of Molecular Sciences 11, no. 9: 3323-3333. https://doi.org/10.3390/ijms11093323