Sequential Events in the Irreversible Thermal Denaturation of Human Brain-Type Creatine Kinase by Spectroscopic Methods
Abstract
:1. Introduction
2. Results and Discussion
2.1. Thermal Denaturation of hBBCK Is Calorimetrically Irreversible
2.2. hBBCK Thermal Denaturation Monitored by CD Spectroscopy
2.3. hBBCK Thermal Denaturation Monitored by Intrinsic Fluorescence Spectroscopy
3. Experimental Section
3.1. Materials
3.2. Protein Expression and Purification
3.3. Activity Assay
3.4. DSC Experiments
3.5. Spectroscopy
4. Conclusions
Acknowledgments
References
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Gao, Y.-S.; Su, J.-T.; Yan, Y.-B. Sequential Events in the Irreversible Thermal Denaturation of Human Brain-Type Creatine Kinase by Spectroscopic Methods. Int. J. Mol. Sci. 2010, 11, 2584-2596. https://doi.org/10.3390/ijms11072584
Gao Y-S, Su J-T, Yan Y-B. Sequential Events in the Irreversible Thermal Denaturation of Human Brain-Type Creatine Kinase by Spectroscopic Methods. International Journal of Molecular Sciences. 2010; 11(7):2584-2596. https://doi.org/10.3390/ijms11072584
Chicago/Turabian StyleGao, Yan-Song, Jing-Tan Su, and Yong-Bin Yan. 2010. "Sequential Events in the Irreversible Thermal Denaturation of Human Brain-Type Creatine Kinase by Spectroscopic Methods" International Journal of Molecular Sciences 11, no. 7: 2584-2596. https://doi.org/10.3390/ijms11072584