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Int. J. Mol. Sci. 2010, 11(5), 2010-2016;

Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.)

Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, Karaj, Iran
Iranain National Gene Bank, Mahdasht Road, Karaj, Iran
Author to whom correspondence should be addressed.
Received: 11 March 2010 / Revised: 17 April 2010 / Accepted: 27 April 2010 / Published: 3 May 2010
(This article belongs to the Section Biochemistry)
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Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. These new markers should allow studies of the population structure and genetic diversity of pomegranate to be performed in the future. View Full-Text
Keywords: Punica granatum; microsatellite; pomegranate; SSR Punica granatum; microsatellite; pomegranate; SSR
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Pirseyedi, S.M.; Valizadehghan, S.; Mardi, M.; Ghaffari, M.R.; Mahmoodi, P.; Zahravi, M.; Zeinalabedini, M.; Nekoui, S.M.K. Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.). Int. J. Mol. Sci. 2010, 11, 2010-2016.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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