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Int. J. Mol. Sci. 2010, 11(11), 4771-4781;

Identification of Compounds in the Essential Oil of Nutmeg Seeds (Myristica fragrans Houtt.) That Inhibit Locomotor Activity in Mice

Faculty of Pharmacy, Universitas Padjadjaran, Jl KM 21.5 Bandung-Sumedang, Jatinangor, Indonesia
Department of Food Technology and Human Nutrition, Bogor Agricultural University, Jl. Darmaga, Bogor, Indonesia
Author to whom correspondence should be addressed.
Received: 11 October 2010 / Revised: 8 November 2010 / Accepted: 15 November 2010 / Published: 23 November 2010
(This article belongs to the Special Issue Advances in Molecular Toxicology)
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The present study was designed to evaluate the inhibitory effect of nutmeg (Myristica fragrans Houtt.) seed essential oil on the locomotor activity of mice in a wheel cage. Active compounds in the essential oil were identified by off-line solid phase extraction (SPE-C18) and GC/MS analysis. The essential oil was administered by inhalation at doses of 0.1, 0.3, and 0.5 mL/cage. The results showed that inhalation of nutmeg seed essential oil at a dose of 0.5 mL/cage decreased locomotion by 68.62%; and inhalation of 0.1 and 0.3 mL/cage inhibited locomotion by 62.81% and 65.33%, respectively. Generally, larger doses and longer administrations of nutmeg seed essential oil exhibited greater locomotor inhibition. Subsequently, the plasma concentrations of essential oil compounds were measured. The most concentrated compound in the plasma was myristicin. Half an hour after the addition of 1 mL/cage of nutmeg seed oil, the plasma concentration of myristicin was 3.7 mg/mL; one and two hours after the addition, the blood levels of myristicin were 5.2 mg/mL and 7.1 mg/mL, respectively. Other essential oil compounds identified in plasma were safrole (two-hour inhalation: 1.28 mg/mL), 4‑terpineol (half-hour inhalation: 1.49 mg/mL, one-hour inhalation: 2.95 mg/mL, two-hour inhalation: 6.28 mg/mL) and fatty esters. The concentrations of the essential oil compounds in the blood plasma were relatively low (mg/mL or ppm). In conclusion, the volatile compounds of nutmeg seed essential oil identified in the blood plasma may correlate with the locomotor-inhibiting properties of the oil when administered by inhalation. View Full-Text
Keywords: nutmeg (Myristica fragrans Houtt.) seeds; essential oils; inhalation; GC-MS nutmeg (Myristica fragrans Houtt.) seeds; essential oils; inhalation; GC-MS
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Muchtaridi; Subarnas, A.; Apriyantono, A.; Mustarichie, R. Identification of Compounds in the Essential Oil of Nutmeg Seeds (Myristica fragrans Houtt.) That Inhibit Locomotor Activity in Mice. Int. J. Mol. Sci. 2010, 11, 4771-4781.

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