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Open AccessArticle

Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data

Laboratory of Biothermodynamics and Drug Design / Institute of Biotechnology, Graičiūno 8, Vilnius, LT-02241, Lithuania
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Int. J. Mol. Sci. 2009, 10(6), 2752-2762; https://doi.org/10.3390/ijms10062752
Received: 19 May 2009 / Revised: 1 June 2009 / Accepted: 15 June 2009 / Published: 18 June 2009
(This article belongs to the Special Issue Isothermal Titration Calorimetry)
Current Isothermal Titration Calorimetry (ITC) data in the literature have relatively high errors in the measured enthalpies of protein-ligand binding reactions. There is a need for universal validation standards for titration calorimeters. Several inorganic salt co-precipitation and buffer protonation reactions have been suggested as possible enthalpy standards. The performances of several commercial calorimeters, including the VP-ITC, ITC200, and Nano ITC-III, were validated using these suggested standard reactions. View Full-Text
Keywords: titration calorimetry; enthalpy; protein-ligand binding; thermodynamics; carbonic anhydrase titration calorimetry; enthalpy; protein-ligand binding; thermodynamics; carbonic anhydrase
MDPI and ACS Style

Baranauskienė, L.; Petrikaitė, V.; Matulienė, J.; Matulis, D. Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data. Int. J. Mol. Sci. 2009, 10, 2752-2762.

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