Detection of Retinoic Acid in Cosmetics Using Reactive Paper Spray Ionization Mass Spectrometry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Online Derivatization Reaction
2.2. Optimization of Derivatization Conditions
2.3. Optimization of the Drying Time for Paper Spray MS
2.4. Sensitivity and Linearity
2.5. Precision of the Experiment
2.6. Sample Recovery Experiment
2.7. Complex Matrix Sample Detection
3. Materials and Methods
3.1. Materials and Reagents
3.2. Instruments
3.3. Sample Preparation
3.4. Derivative Reaction
3.5. MS Conditions
3.6. Derivatization of Retinoic Acid Using Paper Spray MS
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
LC | Liquid chromatography |
MS | Mass spectrometry |
TEA | Triethylamine |
DMPI | N,N′-dimethylpiperazine |
HATU | 2-(7-Azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate |
HBTU | O-Benzotriazole-N,N,N′,N′-tetramethyluronium hexafluorophosphate |
PSI-MS | Paper spray ionization mass spectrometry |
AMS | ambient ionization mass spectrometry |
RPSI-MS | Reactive Paper Spray Ionization Mass Spectrometry |
LOD | limit of detection |
LOQ | limit of quantification |
HPLC | High Performance Liquid Chromatography |
AJS ESI | Agilent Jet Stream Electrospray Ionization |
CE | collision energy |
UHPLC–TOF–MS | Ultra-High Performance Liquid Chromatography—Time of Flight—Mass Spectrometry |
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Chemical Compound | Added (μg·mL−1) | Found (μg·mL−1) | Recovery Rate (%) | Average Recovery Rate (%) | RSD (%) (N = 3) |
---|---|---|---|---|---|
Retinoic acid | 0.001 | 0.0009789 | 97.89 | 96.29 | 1.77 |
0.0009449 | 94.49 | ||||
0.0009649 | 96.49 | ||||
0.01 | 0.01039 | 103.9 | 100.9 | 4.25 | |
0.009623 | 96.23 | ||||
0.01025 | 102.5 | ||||
0.1 | 0.09847 | 98.47 | 98.78 | 1.51 | |
0.09746 | 97.46 | ||||
0.1004 | 100.4 |
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Bao, Y.; Wang, C.; Zhang, N.; Li, J.; Yuan, S.; Yu, L.; Di, B.; Liu, Y. Detection of Retinoic Acid in Cosmetics Using Reactive Paper Spray Ionization Mass Spectrometry. Molecules 2025, 30, 1906. https://doi.org/10.3390/molecules30091906
Bao Y, Wang C, Zhang N, Li J, Yuan S, Yu L, Di B, Liu Y. Detection of Retinoic Acid in Cosmetics Using Reactive Paper Spray Ionization Mass Spectrometry. Molecules. 2025; 30(9):1906. https://doi.org/10.3390/molecules30091906
Chicago/Turabian StyleBao, Yuzhang, Chenyu Wang, Na Zhang, Jie Li, Song Yuan, Liju Yu, Bin Di, and Yang Liu. 2025. "Detection of Retinoic Acid in Cosmetics Using Reactive Paper Spray Ionization Mass Spectrometry" Molecules 30, no. 9: 1906. https://doi.org/10.3390/molecules30091906
APA StyleBao, Y., Wang, C., Zhang, N., Li, J., Yuan, S., Yu, L., Di, B., & Liu, Y. (2025). Detection of Retinoic Acid in Cosmetics Using Reactive Paper Spray Ionization Mass Spectrometry. Molecules, 30(9), 1906. https://doi.org/10.3390/molecules30091906