Visualization and Comparison of the Level of Apurinic/Apyrimidinic Endonuclease 1 in Live Normal/Cancerous and Neuron Cells with a Fluorescent Nanoprobe
Abstract
:1. Introduction
2. Results and Discussion
2.1. Construction and Characterization of the DNA/RNA Hybrid Fluorescent Nanoprobe (D0/R-Nanoprobe)
2.2. Fluorescence Imaging of APE1 Activity in Live MCF-7 and MCF-10A Cells
2.3. Time-Lapse Fluorescence Imaging of the APE1 Activity in PC-12 Live Cells with the D0/R-Nanoprobes
2.4. Real-Time Monitoring of the Changes of APE1 Level in PC-12 under Severe Attack by ROS
3. Materials and Methods
3.1. Chemical Reagents and Materials
3.2. Synthesis of DNA/RNA Hybrid Nanoprobes
3.3. Sensitivity and Selectivity Measurement of D0/R-Nanoprobes
3.4. Intracellular Uptake of D0/R-Nanoprobes for Fluorescence Imaging
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lu, P.; Cao, X.; Zheng, J.; Sun, Y.; Tang, Z.; Zhao, M. Visualization and Comparison of the Level of Apurinic/Apyrimidinic Endonuclease 1 in Live Normal/Cancerous and Neuron Cells with a Fluorescent Nanoprobe. Molecules 2023, 28, 3935. https://doi.org/10.3390/molecules28093935
Lu P, Cao X, Zheng J, Sun Y, Tang Z, Zhao M. Visualization and Comparison of the Level of Apurinic/Apyrimidinic Endonuclease 1 in Live Normal/Cancerous and Neuron Cells with a Fluorescent Nanoprobe. Molecules. 2023; 28(9):3935. https://doi.org/10.3390/molecules28093935
Chicago/Turabian StyleLu, Peng, Xiangjian Cao, Jinghui Zheng, Ying Sun, Ziyu Tang, and Meiping Zhao. 2023. "Visualization and Comparison of the Level of Apurinic/Apyrimidinic Endonuclease 1 in Live Normal/Cancerous and Neuron Cells with a Fluorescent Nanoprobe" Molecules 28, no. 9: 3935. https://doi.org/10.3390/molecules28093935