(-)-Epigallocatechin-3-Gallate Attenuates the Adverse Reactions Triggered by Selenium Nanoparticles without Compromising Their Suppressing Effect on Peritoneal Carcinomatosis in Mice Bearing Hepatocarcinoma 22 Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effects of SeNPs on Peritoneal Carcinomatosis in Mice Bearing H22 Cells
2.2. EGCG Enhances the Production of SeNP-Induced ROS in H22 Cell Suspension
2.3. EGCG Reduces the Diarrhea Proportion Caused by SeNPs without Compromising Their Suppressing Efficacy on Peritoneal Carcinomatosis in Mice
2.4. Effect of EGCG on Peritoneal Carcinomatosis in Mice Bearing H22 Cells
2.5. EGCG Protects against SeNP-Triggered Systemic Toxicity via Restoring Antioxidant Defense in Healthy Mice
3. Materials and Methods
3.1. Chemicals and Materials
3.2. Preparation and Characterization of SeNPs
3.3. Animals and H22 Model Mice
3.4. H22 Cell Collection and Count
3.5. ROS Measurement
3.6. Determination of Enzyme Activities
3.7. Determination of Selenium Level in Tissues
3.8. EGCG Measurement in H22 Cells and Tissues
3.9. Western Blot Analysis
3.10. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ban, Q.; Chi, W.; Wang, X.; Wang, S.; Hai, D.; Zhao, G.; Zhao, Q.; Granato, D.; Huang, X. (-)-Epigallocatechin-3-Gallate Attenuates the Adverse Reactions Triggered by Selenium Nanoparticles without Compromising Their Suppressing Effect on Peritoneal Carcinomatosis in Mice Bearing Hepatocarcinoma 22 Cells. Molecules 2023, 28, 3904. https://doi.org/10.3390/molecules28093904
Ban Q, Chi W, Wang X, Wang S, Hai D, Zhao G, Zhao Q, Granato D, Huang X. (-)-Epigallocatechin-3-Gallate Attenuates the Adverse Reactions Triggered by Selenium Nanoparticles without Compromising Their Suppressing Effect on Peritoneal Carcinomatosis in Mice Bearing Hepatocarcinoma 22 Cells. Molecules. 2023; 28(9):3904. https://doi.org/10.3390/molecules28093904
Chicago/Turabian StyleBan, Qiuyan, Wenjing Chi, Xiaoxiao Wang, Shiqiong Wang, Dan Hai, Guangshan Zhao, Qiuyan Zhao, Daniel Granato, and Xianqing Huang. 2023. "(-)-Epigallocatechin-3-Gallate Attenuates the Adverse Reactions Triggered by Selenium Nanoparticles without Compromising Their Suppressing Effect on Peritoneal Carcinomatosis in Mice Bearing Hepatocarcinoma 22 Cells" Molecules 28, no. 9: 3904. https://doi.org/10.3390/molecules28093904