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Article

Insights into the Oxidative Stress Alleviation Potential of Enzymatically Prepared Dendrobium officinale Polysaccharides

by
Yingqi Tang
1,
Xiong Zhang
2,
Yudan Lin
1,
Jiehan Sun
1,
Shihao Chen
3,
Weimin Wang
1 and
Jia Li
1,4,*
1
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
2
Hangzhou Zaoxianyibu Food Technology Co., Ltd., Hangzhou 310018, China
3
Hangzhou Jiuxian Biotechnology Co., Ltd., Hangzhou 311618, China
4
Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, Hangzhou 310018, China
*
Author to whom correspondence should be addressed.
Molecules 2023, 28(7), 3071; https://doi.org/10.3390/molecules28073071
Submission received: 4 March 2023 / Revised: 23 March 2023 / Accepted: 27 March 2023 / Published: 30 March 2023
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Abstract

(1) Background: The extraction parameters can dramatically alter the extraction rate and biological activity of polysaccharides. (2) Methods: Here, an enzyme-assisted extraction (EAE) was employed to extract D. officinale polysaccharides (DOPs), and its optimal extraction conditions were established by single-factor and Box–Behnken design (BBD) experiments. Further, on the basis of in vitro antioxidant capacity, the paraquat (PQ)-induced oxidative stress of Caenorhabditis elegans (C. elegans) was chosen as a research model to explore the antioxidant activity of DOPs. (3) Results: The results showed that the extraction yield of DOPs reached 48.66% ± 1.04% under the optimal condition. In vitro experiments had shown that DOPs have considerable ABTS+ radical scavenging capacity (EC50 = 7.27 mg/mL), hydroxyl radical scavenging capacity (EC50 = 1.61 mg/mL), and metal chelating power (EC50 = 8.31 mg/mL). Furthermore, in vivo experiments indicated that DOPs (0.25 mg/mL) significantly prolonged the lifespan, increased antioxidant enzyme activity, and upregulated the expression of daf-16 (>5.6-fold), skn-1 (>5.2-fold), and sir-2.1 (>2.3-fold) of C. elegans. (4) Conclusions: DOPs can be efficiently extracted by EAE and are effective in the reduction of oxidative stress levels in C. elegans.
Keywords: Dendrobium officinale polysaccharide; enzyme-assisted extraction; oxidative stress resistance Dendrobium officinale polysaccharide; enzyme-assisted extraction; oxidative stress resistance
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MDPI and ACS Style

Tang, Y.; Zhang, X.; Lin, Y.; Sun, J.; Chen, S.; Wang, W.; Li, J. Insights into the Oxidative Stress Alleviation Potential of Enzymatically Prepared Dendrobium officinale Polysaccharides. Molecules 2023, 28, 3071. https://doi.org/10.3390/molecules28073071

AMA Style

Tang Y, Zhang X, Lin Y, Sun J, Chen S, Wang W, Li J. Insights into the Oxidative Stress Alleviation Potential of Enzymatically Prepared Dendrobium officinale Polysaccharides. Molecules. 2023; 28(7):3071. https://doi.org/10.3390/molecules28073071

Chicago/Turabian Style

Tang, Yingqi, Xiong Zhang, Yudan Lin, Jiehan Sun, Shihao Chen, Weimin Wang, and Jia Li. 2023. "Insights into the Oxidative Stress Alleviation Potential of Enzymatically Prepared Dendrobium officinale Polysaccharides" Molecules 28, no. 7: 3071. https://doi.org/10.3390/molecules28073071

APA Style

Tang, Y., Zhang, X., Lin, Y., Sun, J., Chen, S., Wang, W., & Li, J. (2023). Insights into the Oxidative Stress Alleviation Potential of Enzymatically Prepared Dendrobium officinale Polysaccharides. Molecules, 28(7), 3071. https://doi.org/10.3390/molecules28073071

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