Ribosome Biogenesis Regulator 1 Homolog (RRS1) Promotes Cisplatin Resistance by Regulating AEG-1 Abundance in Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. RRS1 Was Highly Expressed in Cisplatin-Resistant Breast Cancer Cells and Regulated Chemosensitivity
2.2. RRS1 Knockdown Reduced the Proliferative Rates of MCF-7/DDP Cells and Sensitized Them to Cisplatin
2.3. AEG-1 Might Participate in Cisplatin Resistance Mediated by RRS1
2.4. RRS1 Blocked AEG-1 Ubiquitination and Proteasome Degradation
2.5. Cisplatin Induced RRS1 Overexpression In Vivo
3. Materials and Methods
3.1. Cell Line Authentication
3.2. Cell Culture and Chemical Reagents
3.3. Lentivirus Transfection
3.4. Quantitative Reverse Transcription PCR (RT-qPCR)
3.5. Cisplatin Sensitivity Analysis
3.6. Cell Proliferation Assay
3.7. Cell Cycle and Apoptosis
3.8. TUNEL Staining
3.9. Co-Immunoprecipitation (Co-IP)
3.10. Protein Stability Assay
3.11. Xenograft Assay
3.12. Western Blotting
3.13. Statistical Analysis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Song, J.; Peng, C.; Wang, R.; Hua, Y.; Wu, Q.; Deng, L.; Cao, Y.; Zhang, L.; Hou, L. Ribosome Biogenesis Regulator 1 Homolog (RRS1) Promotes Cisplatin Resistance by Regulating AEG-1 Abundance in Breast Cancer Cells. Molecules 2023, 28, 2939. https://doi.org/10.3390/molecules28072939
Song J, Peng C, Wang R, Hua Y, Wu Q, Deng L, Cao Y, Zhang L, Hou L. Ribosome Biogenesis Regulator 1 Homolog (RRS1) Promotes Cisplatin Resistance by Regulating AEG-1 Abundance in Breast Cancer Cells. Molecules. 2023; 28(7):2939. https://doi.org/10.3390/molecules28072939
Chicago/Turabian StyleSong, Junying, Cuixiu Peng, Runze Wang, Yanan Hua, Qinglan Wu, Lin Deng, Yi Cao, Li Zhang, and Lin Hou. 2023. "Ribosome Biogenesis Regulator 1 Homolog (RRS1) Promotes Cisplatin Resistance by Regulating AEG-1 Abundance in Breast Cancer Cells" Molecules 28, no. 7: 2939. https://doi.org/10.3390/molecules28072939