Synthetic Glabridin Derivatives Inhibit LPS-Induced Inflammation via MAPKs and NF-κB Pathways in RAW264.7 Macrophages
Abstract
:1. Introduction
2. Results
2.1. Effects of Compounds on LPS-Induced NO and PGE2 Production
2.2. Effects of Compounds on LPS-Induced Cytokines
2.3. Effects of Compounds on NF-κB Signaling Pathway
2.4. Effects of Compounds on LPS-Induced MAPK Signaling
2.5. Effects of Compounds on HO-1 Induction and Nrf2 Signaling
2.6. Effects of Compounds on MAPK Signaling Involved in HO-1 Induction
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture and Viability Assay
4.3. NO Production
4.4. PGE2 Assay
4.5. Western Blot Analysis
4.6. Preparation of Cytosolic and Nuclear Fractions
4.7. DNA-Binding Activity of NF-κB
4.8. Quantitative Real-Time Reverse Transcriptase PCR (qRT-PCR) Assay
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compound | NO (IC50, μM) | PGE2 (IC50, μM) | Cytokine mRNA Expression Inhibition | NF-κB Pathway Inhibition | MAPK Pathway Inhibition | HO-1 Induction by MAPK |
---|---|---|---|---|---|---|
Glabridin | 9.36 | 7.09 | IL-1β, IL-6, TNF-α | p-IκB-α | JNK, p38 | p38, ERK |
HSG4112 | 6.79 | 3.55 | ERK | |||
(S)-HSG4112 | 3.85 | 2.37 | JNK, p38 | |||
HGR4113 | 11.32 | 1.64 | JNK |
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Shin, J.; Choi, L.S.; Jeon, H.J.; Lee, H.M.; Kim, S.H.; Kim, K.-W.; Ko, W.; Oh, H.; Park, H.S. Synthetic Glabridin Derivatives Inhibit LPS-Induced Inflammation via MAPKs and NF-κB Pathways in RAW264.7 Macrophages. Molecules 2023, 28, 2135. https://doi.org/10.3390/molecules28052135
Shin J, Choi LS, Jeon HJ, Lee HM, Kim SH, Kim K-W, Ko W, Oh H, Park HS. Synthetic Glabridin Derivatives Inhibit LPS-Induced Inflammation via MAPKs and NF-κB Pathways in RAW264.7 Macrophages. Molecules. 2023; 28(5):2135. https://doi.org/10.3390/molecules28052135
Chicago/Turabian StyleShin, Jaejin, Leo Sungwong Choi, Hyun Ju Jeon, Hyeong Min Lee, Sang Hyo Kim, Kwan-Woo Kim, Wonmin Ko, Hyuncheol Oh, and Hyung Soon Park. 2023. "Synthetic Glabridin Derivatives Inhibit LPS-Induced Inflammation via MAPKs and NF-κB Pathways in RAW264.7 Macrophages" Molecules 28, no. 5: 2135. https://doi.org/10.3390/molecules28052135