Sensitive and Label-Free Colorimetric Detection of Glyphosate Based on the Suppression Peroxidase-Mimicking Activity of Cu(II) Ions
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cu(II) Ions Display Peroxidase Catalytic Activity
2.2. Glyp Can Suppress Peroxidase-Mimicking Activity of Cu(II) Ions
2.3. Optimization of Experimental Conditions
2.4. Analytical Performance of Developed Strategy
2.5. Glyp Detection in Real Samples
3. Materials and Methods
3.1. Chemicals and Instrument
3.2. Cu(II) Ions as a Peroxidase
3.3. Optimization of the Detection Conditions
3.4. Enzyme Kinetics Testing of Cu(II) Ions
3.5. Mechanism of Cu(II) Catalyzed Reaction
3.6. Detection of Glyp
3.7. Selectivity Study for Glyp Detection
3.8. Determination of Glyp in Real Samples
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Material | Detection Mode | Detection Range (μg/mL) | LOD (ng/mL) | Reference |
---|---|---|---|---|
Gold electrode | Electrochemical | -- | 270.51 | 30 |
Copper (II)- pyrocatechol violet based GlyPKit | Colorimetry | 1.69~10.13 | 422 | 31 |
Silver Nanoparticles | UV-Vis | -- | 1000 | 32 |
Raman transduction | 1300 | |||
Red-Emitting Polymerizable Guanidinium Dyes | Fluorometry | -- | 101.44 | 33 |
Molecularly imprinted polypyrrole | Voltametric | 0.4~1.2 | 92 | 34 |
The suppression peroxidase-mimicking activity of Cu2+ | Colorimetry | 2~20 | 95.6 | This work |
Method | Added (μM) | Found (μM) | Recovery (%) | RSD (n = 3) |
---|---|---|---|---|
This work | 2.5 | 2.615 | 104.60 | 5.13% |
3.0 | 2.885 | 96.17 | 2.36% | |
6.0 | 6.307 | 105.12 | 3.58% | |
HPLC | 2.5 | 2.530 | 101.20 | 0.86% |
3.0 | 2.986 | 99.53 | 0.79% | |
6.0 | 6.013 | 100.22 | 0.82% |
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Li, Q.; Guo, Y.; He, X.; Li, G. Sensitive and Label-Free Colorimetric Detection of Glyphosate Based on the Suppression Peroxidase-Mimicking Activity of Cu(II) Ions. Molecules 2023, 28, 4630. https://doi.org/10.3390/molecules28124630
Li Q, Guo Y, He X, Li G. Sensitive and Label-Free Colorimetric Detection of Glyphosate Based on the Suppression Peroxidase-Mimicking Activity of Cu(II) Ions. Molecules. 2023; 28(12):4630. https://doi.org/10.3390/molecules28124630
Chicago/Turabian StyleLi, Qing, Yumeng Guo, Xiangyi He, and Guangli Li. 2023. "Sensitive and Label-Free Colorimetric Detection of Glyphosate Based on the Suppression Peroxidase-Mimicking Activity of Cu(II) Ions" Molecules 28, no. 12: 4630. https://doi.org/10.3390/molecules28124630