Diet Restriction Impact on High-Fat-Diet-Induced Obesity by Regulating Mitochondrial Cardiolipin Biosynthesis and Remodeling
Abstract
:1. Introduction
2. Results
2.1. Mitochondria Enrichment and Identification
2.2. Physiological and Biochemical Parameters
2.3. Identification of Mitochondrial CLs in the Liver
2.4. Distribution of Mitochondrial CLs in the Liver
2.5. Targeted Metabolomic Analysis of Mitochondrial CLs in the Liver
2.6. The Screening of Mitochondrial CLs Related to DR Ameliorate Obesity
2.7. The Biosynthesis and Remodeling Gene Expression of CLs
3. Discussion
4. Materials and Methods
4.1. Chemicals and Materials
4.2. Animals and DR
4.3. HE Staining and Morphometric Analysis
4.4. Biochemical Analysis
4.5. Isolation and Purity Verification of Mitochondria
4.5.1. Isolation of Mitochondria
4.5.2. Western Blot Analysis
4.5.3. Transmission Electron Microscopy
4.6. Extraction of CL from Isolated Liver Mitochondria
4.7. Identification of Mitochondrial CLs by UHPLC-QTOF-MS/MS
4.8. Targeted Metabolomic Analysis
4.9. Real-Time Polymerase Chain Reaction (RT-PCR) Analysis
4.10. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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ND | HFD | 0 DR | 20 DR | 40 DR | 60 DR | |
---|---|---|---|---|---|---|
Body weight (g) | 42.86 ± 4.49 ** | 50.63 ± 3.06 | 46.24 ± 2.46 ** | 38.30 ± 4.15 *** | 31.26 ± 3.43 ***,# | 29.16 ± 0.95 ***,### |
BMI at the first stage | 309.15 ± 3.60 ** | 315.41 ± 2.00 | 316.58 ± 3.36 ## | 316.16 ± 3.56 ## | 316.65 ± 3.39 ## | 316.60 ± 2.88 ## |
BMI at the second stage | 313.73 ± 6.86 ** | 330.18 ± 4.66 | 314.65 ± 9.41 *** | 299.91 ± 7.82 ***,## | 288.66 ± 5.99 ***,### | 294.04 ± 3.96 ***,### |
Liver weight | 1.94 ± 0.23 * | 2.27 ± 0.26 | 1.77 ± 0.14 ***,# | 1.85 ± 0.29 ** | 1.47 ± 0.16 ***,## | 1.42 ± 0.24 ***,## |
TC (mmol L−1) | 2.65 ± 0.29 *** | 6.74 ± 0.91 | 3.23 ± 0.27 ***,## | 2.54 ± 0.45 *** | 2.88 ± 0.79 *** | 3.22 ± 0.47 ***,# |
HDL-C (mmol L−1) | 2.05 ± 0.27 *** | 3.20 ± 0.44 | 2.45 ± 0.33 **,# | 1.61 ± 0.35 ***,# | 1.65 ± 0.37 ***,# | 1.63 ± 0.21 ***,## |
LDL-C (mmol L−1) | 0.45 ± 0.13 *** | 1.26 ± 0.27 | 0.49 ± 0.08 *** | 0.34 ± 0.12 *** | 0.38 ± 0.18 *** | 0.47 ± 0.13 *** |
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Li, Q.; Lin, Y.; Xu, J.; Liu, Y.; Jing, Y.; Huang, R.; Song, C.; Zhang, L.; Jin, S. Diet Restriction Impact on High-Fat-Diet-Induced Obesity by Regulating Mitochondrial Cardiolipin Biosynthesis and Remodeling. Molecules 2023, 28, 4522. https://doi.org/10.3390/molecules28114522
Li Q, Lin Y, Xu J, Liu Y, Jing Y, Huang R, Song C, Zhang L, Jin S. Diet Restriction Impact on High-Fat-Diet-Induced Obesity by Regulating Mitochondrial Cardiolipin Biosynthesis and Remodeling. Molecules. 2023; 28(11):4522. https://doi.org/10.3390/molecules28114522
Chicago/Turabian StyleLi, Qiaoyu, Yuqi Lin, Jinlin Xu, Yukun Liu, Yuxuan Jing, Rongzeng Huang, Chengwu Song, Lijun Zhang, and Shuna Jin. 2023. "Diet Restriction Impact on High-Fat-Diet-Induced Obesity by Regulating Mitochondrial Cardiolipin Biosynthesis and Remodeling" Molecules 28, no. 11: 4522. https://doi.org/10.3390/molecules28114522