Dual Spinneret Electrospun Polyurethane/PVA-Gelatin Nanofibrous Scaffolds Containing Cinnamon Essential Oil and Nanoceria for Chronic Diabetic Wound Healing: Preparation, Physicochemical Characterization and In-Vitro Evaluation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Cerium Oxide Nanoparticles (nCeO2)
2.3. Physicochemical Characterization of Nanoceria
2.4. Preparation of Electrospinning Solutions
2.5. Fabrication of PU-EO/PVA-Gel- nCeO2 Electrospun Membranes
2.6. Mechanical, Chemical, and Morphological Properties
2.7. Biodegradability and Swelling
2.8. Antibacterial Activity
2.9. Cytotoxic Study
2.9.1. MTT Cytotoxicity Assay
2.9.2. Fluorescence Imaging
2.10. Statistical Analysis
3. Results and Discussion
3.1. Characterization of nCeO2
3.2. SEM of CEO/nCeO2 Loaded PU/PVA-Gel NFs
3.3. Swelling and Biodegradability
3.4. Mechanical Properties
3.5. FTIR Study
3.6. DSC Analysis
3.7. Antibacterial Activity
3.8. Cytobiocompatability
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample No. | PU Layer Solution | PVA-Gel Layer Solution | Nanofibers Sample Code | ||||
---|---|---|---|---|---|---|---|
PU (wr%) | CEO (v/v%) | PVA (wt%) | Gel (wt%) | PVA/Gel | nCeO2 (w/w%) | ||
PU | 12 | - | - | - | - | - | PU |
S1 | 12 | - | 12 | 10 | 7/3 | - | Pure-S1 |
S2 | 12 | - | 12 | 10 | 7/3 | 0.5 | CEO/nCeO2-S2 |
S3 | 12 | 2.25 | 12 | 10 | 7/3 | 0.5 | CEO/nCeO2-S3 |
S4 | 12 | 4.5 | 12 | 10 | 7/3 | 0.5 | CEO/nCeO2-S4 |
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Hussein, M.A.M.; Gunduz, O.; Sahin, A.; Grinholc, M.; El-Sherbiny, I.M.; Megahed, M. Dual Spinneret Electrospun Polyurethane/PVA-Gelatin Nanofibrous Scaffolds Containing Cinnamon Essential Oil and Nanoceria for Chronic Diabetic Wound Healing: Preparation, Physicochemical Characterization and In-Vitro Evaluation. Molecules 2022, 27, 2146. https://doi.org/10.3390/molecules27072146
Hussein MAM, Gunduz O, Sahin A, Grinholc M, El-Sherbiny IM, Megahed M. Dual Spinneret Electrospun Polyurethane/PVA-Gelatin Nanofibrous Scaffolds Containing Cinnamon Essential Oil and Nanoceria for Chronic Diabetic Wound Healing: Preparation, Physicochemical Characterization and In-Vitro Evaluation. Molecules. 2022; 27(7):2146. https://doi.org/10.3390/molecules27072146
Chicago/Turabian StyleHussein, Mohamed Ahmed Mohamady, Oguzhan Gunduz, Ali Sahin, Mariusz Grinholc, Ibrahim Mohamed El-Sherbiny, and Mosaad Megahed. 2022. "Dual Spinneret Electrospun Polyurethane/PVA-Gelatin Nanofibrous Scaffolds Containing Cinnamon Essential Oil and Nanoceria for Chronic Diabetic Wound Healing: Preparation, Physicochemical Characterization and In-Vitro Evaluation" Molecules 27, no. 7: 2146. https://doi.org/10.3390/molecules27072146