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Variability in the Chemical Composition of Myrcia sylvatica (G. Mey) DC. Essential Oils Growing in the Brazilian Amazon

Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pará, Belém 66075-110, Brazil
Laboratório de Química dos Produtos Naturais, Universidade do Estado do Pará, Belém 66087-662, Brazil
Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém 66087-662, Brazil
Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA
Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Pará, Belém 66075-900, Brazil
Programa de Pós-Graduação em Química, Universidade Federal do Maranhão, São Luís 64080-040, Brazil
Departamento de Ciências Naturais, Centro de Ciência Sociais e Educação, Universidade do Estado do Pará, Belém 66050-540, Brazil
Author to whom correspondence should be addressed.
Molecules 2022, 27(24), 8975;
Received: 12 November 2022 / Revised: 12 December 2022 / Accepted: 13 December 2022 / Published: 16 December 2022
(This article belongs to the Special Issue Essential Oils II)


Myrcia sylvatica (G. Mey) DC. is known as “insulin plant” because local communities use the infusions of various organs empirically to treat diabetes. The leaves of seven specimens of Myrcia sylvatica (Msy-01 to Msy-07) were collected in the Brazilian Amazon. Furthermore, the essential oils were extracted by hydrodistillation and analyzed by gas chromatography coupled to mass spectrometry, and their chemical compositions were submitted to multivariate analysis (Principal Component Analysis and Hierarchical Cluster Analysis). The multivariate analysis displayed the formation of four chemical profiles (chemotypes), described for the first time as follows: chemotype I (specimen Msy-01) was characterized by germacrene B (24.5%), γ-elemene (12.5%), and β-caryophyllene (10.0%); chemotype II (specimens Msy-03, -06 and -07) by spathulenol (11.1–16.0%), germacrene B (7.8–20.7%), and γ-elemene (2.9–7.6%); chemotype III (Msy-04 and -05) by spathulenol (9.8–10.1%), β-caryophyllene (2.5–10.1%), and δ-cadinene (4.8-5.6%); and chemotype IV, (Msy-02) by spathulenol (13.4%), caryophyllene oxide (15.0%), and α-cadinol (8.9%). There is a chemical variability in the essential oils of Myrcia sylvatica occurring in the Amazon region.

Graphical Abstract

1. Introduction

The Myrcia genus has about 800 species distributed from Central to Tropical America [1]. In addition, it is considered one of the most taxonomically and morphologically complex homogeneous genera of the Myrtaceae family [2], including in the Myrtales order, Rosideas clade, and Malvideas sub-clade [3]. The Amazon rainforest, despite comprising a low diversity of Myrcia spp., was an important region in the biogeographic history of this genus because evidence indicates that it participated in the diversification of ancestral lineages [4].
Myrcia species have great ecological relevance, as their fruits are a food source for ants, birds, and mammals, and their flowers are attractive to pollinators, such as bees. These ecological relationships are responsible for promoting the conservation of the diversity of this genus [5]. In addition, Myrcia species have economic, nutritional [6], and medicinal importance [7].
Myrcia species can be recognized in the field by the sweet aroma emanating from the leaves, flowers, and fruits, in addition to generally appearing as shrubs with leaves elliptical-co-lanceolate; apex long-acuminate to caudate; inflorescences in panicles; and flowers with deltoid sepals and petals white—rarely yellow—connective with glands of blackish color and stigma hairy at the base [8].
Several species of the Myrcia genus are popularly known as “pedra-ume-caá” or “pedra-hume-caá”, among them Myrcia punicifolia (Kunth) DC., M. speciosa (Amsh.) Mc Vaugh, M. amazonica DC., M. citrifolia (Aubl.) Urb., M. guianensis (Aubl.) DC., M. multiflora (Lam.) DC., M. salicifolia DC., M. sylvatica (G. Mey) DC., and M. uniflora DC. These species are also as known “insulin plant” because local communities use the infusions of various organs of these plants empirically to treat diabetes [9].
Myrcia sylvatica (G. Mey) DC. is also known as “kumate-folha-miúda” or “murtinha”. It is native and non-endemic to Brazil, widely distributed in South America, where it is found from Guyana to Brazil [10]. However, in Brazil, its occurrence is restricted to the phytogeographic domains of the Amazon, Caatinga, and Cerrado [11].
The M. sylvatica essential oil have shown great chemical variability due to intraspecific or seasonal variations [9,12], in addition to antioxidant, anesthetic potential [13] and bactericidal properties [14].
Therefore, in view of the biological potential presented by Myrcia sylvatica, the objective of this work was to investigate the chemical variability of the essential oils of leaves that occur in the Amazon of Pará.

2. Results and Discussion

2.1. Yield and Chemical Composition of the Essential Oils

The seven Myrcia sylvatica wild specimens evaluated in this work showed chemical variability of their essential oils. The oil yield ranged from 0.3 to 0.9%, as shown in Table 1. The quantification and identification of 112 constituents in the analyzed oils represent an average of 81.1% of the total oil content.
Sesquiterpene hydrocarbons (12.5–71.8%) and oxygenated sesquiterpenoids (17.4–71.5%) were predominant in the essential oils. The main compounds (>5%) identified in the oils were the sesquiterpenes with germacrane (germacrene B, 0.3-24.5%; γ-elemene, 0.3–12.5%), aromadendrane (spathulenol, 2.9–16.0%; globulol, 0.0–7.4%; and viridiflorol, 0.8–5.3%), and caryophyllane skeletons (caryophyllene oxide, 0.1–15.0%; and β-caryophyllene, 1.8–10.1%), followed by sesquiterpenes with cadinane skeletons (α-cadinol, 1.7–8.9%; muurola-4,10(14)-dien-1-β-ol, 0.0–5.8%; δ-cadinene, 1.1–5.6%; and epi-α-cadinol, 0.0–5.1%), as shown in Figure 1.
The seasonal and circadian study of essential oil from leaves and fruits of M. sylvatica collected in the municipality of Santarém, state of Pará, indicated that the yield varied from 0.9 to 1.7% [12], values higher than this work. In contrast, the yield of leaf essential oil from this species collected in Carolina, state of Maranhão, was 0.5% [17], the same content presented by the specimen Msy-07.
In the Myrtaceae species essential oils, the predominance of hydrocarbon and oxygenated sesquiterpenes has been evidenced, some of them with biological properties [18,19]. The presence of the sesquiterpene hydrocarbon β-caryophyllene (45.0%) as the major constituent was identified in a M. sylvatica sample collected in Maranhão [17]. Other compounds were also reported as the main compound in oils from Tocantins, among them the oxygenated sesquiterpenes spathulenol (13.8–40.2%) and caryophyllene oxide (5.0–16.6%) [10]. Germacrene B (6.7%) and γ-elemene (10.5%) were identified as the highest content in M. splendens [20].

2.2. Chemical Variability in the Specimens

The Hierarchical Cluster Analysis (HCA, Figure 2) and the Principal Components Analysis (PCA, Figure 3), carried out with the compounds in the highest abundance (> 4.0%) in the essential oils of M. sylvatica, displayed the formation of four groups (chemotypes).
The Principal Components Analysis elucidated 81.5% of the data variability. PC1 explained 42.3% and showed positive correlations with the constituents spathulenol (r = 0.22), caryophyllene oxide (r = 0.33), viridiflorol (r = 0.29), muurola-4,10(14)-dien-1β-ol (r = 0.36), epi-α-murrolol (r = 0.34), and α-cadinol (r = 0.36). The second component explained 22.3% and presented a positive correlation with the compounds β-caryophyllene (r = 0.07), germacrene D (r = 0.43), δ-cadinene (r = 0.48), caryophyllene oxide (r = 0.05), viridiflorol (r = 0.08), α-cadinol (r = 0.19), and epi-α-cadinol (r = 0.47). The third component, PC3, explained 17.0% of the data and explained a positive correlation with the variables β-caryophyllene (r = 0.18), γ-elemene (r = 0.33), germacrene D (r = 0.05), bicyclogermacrene (r = 0.25), germacrene B (r = 0.23), caryophyllene oxide (r = 0.33), viridiflorol (r = 0.23), muurola-4,10(14)-dien-1β-ol (r = 0.25), epi-α-cadinol (r = 0.13), and α-cadinol (r = 0.16).
From this, the oil samples were classified into four chemotypes (chromatogram displayed in Figure A1). Group I (specimen Msy-01) was characterized by germacrene B (24.5%), γ-elemene (12.5%), and β-caryophyllene (10.0%). Group II (Msy-03, -06 and -07 specimens) was characterized by the contents of spathulenol (11.1–16.0%), germacrene B (7.8–20.7%), and γ -elemene (2.9–7.6%). Group III (Msy-04 and -05) showed spathulenol (9.8–10.1%), β-caryophyllene (2.5–10.1%), and δ-cadinene (4.8–5, 6%). Group IV (Msy-02) was characterized by spathulenol (13.4%), caryophyllene oxide (15.0%), and α-cadinol (8.9%).
Three chemical profiles of M. sylvatica samples collected in Tocantins were reported, the first one exhibiting selin-11-en-4α-ol (24.7%), caryophyllene oxide (16.6%), and spathulenol (13.8%) as the main constituents. The second was characterized by cis-calamenene (30.1%), spathulenol (18.7%), and α-calacorene (11.5%), and the third by spathulenol (40.2%) and β-bisabolene (14.7%) [10]. The oxygenated sesquiterpene spathulenol was present in all samples of this work. Saccol et al., analyzing the chemical composition and the anesthetic and antioxidant effects of M. sylvatica essential oil, identified β-selinene (9.96%), cadalene (9.36%), α-calacorene (9.17%), and (Z)-calamene (8.17%) as major compounds [13], which is different from the chemical profiles of this study.
Furthermore, the seasonal and circadian study of a specimen of M. sylvatica from Santarém, Pará, revealed the influence of climatic factors on the chemical composition of the oils of this species, whose main constituents during the collection period were β-selinene (6.2–10.5%), 1-epi-cubenol (5.9–9.8%), cadalene (1.5–6.5%), mustakone (2.7–6.2%), α-calacorene (1.5–6.2%), δ-cadinene (0.7–6.0%), cubenol (2.4–4.6%), trans-calamenene (3.5–6.5%), and caryophyllene oxide (2.5–4.0%) [12]. All these compounds were also present in the oils of the studied M. sylvatica specimens.
In another study, carried out by Silva et al. [14], the chemical composition of fresh and dried leaves of M. sylvatica, also collected in Santarém, exhibited the compounds 1-epi-cubenol (6.9–9.9%), ar-curcumene (1.9–7.6%), cadalene (5.8–7.2%), β-selinene (6.0–7.0%), β-calacorene (5.4–5.5%), cis-calamenene (4.8–5.2%), ar-turmerol (0.0–4.9%), muskatone (3.4–4.4%), δ-cadinene (4.2%), and cubenol (4.2%). Only the constituents ar-curcumene and cis-calamenene were not identified in the samples of this work.
Another specimen collected in Bujaru, Pará state, was rich in (Z)-trans-α-bergamotene (24.6%), followed by α-sinensal (13.4%), (Z)-α-bisabolene (8.3%), trans-α-bisabolene (7.1%), and trans-β-bisabolene (5.1%). These constituents were not identified in the collected specimens [21]. The oil extracted from a specimen collected in the state of Maranhão showed β-caryophyllene (45.9%), hydroxy-(Z)-caryophyllene (10.2%), β-selenene (5.9%), and seline-3,11-diene (5.4%) in higher content [17]. The sesquiterpenes β-caryophyllene and β-selenene were also identified in the oils of the M. sylvatica described in this work.
Essential oils from Myrtaceae species have shown chemical variability, which may be influenced by seasonality, collection site, extraction method, genetics, and plant part [18,22,23]. This variability affects their biological properties and applications; for example, the existence of four Eugenia uniflora chemotypes was reported, and the samples presented different biological potentials related to their chemical profiles [24].
Therefore, among the collected samples, all chemical profiles were described for the first time: Profile I (germacrene B, γ-elemene, and β-caryophyllene), Profile II (spathulenol, germacrene B, and γ-elemene), Profile III (spathulenol, β-caryophyllene, and δ-cadinene), and Profile IV (spathulenol, caryophyllene oxide, and α-cadinol). Thus, added to the eight chemotypes described in the literature, it is possible that there are at least twelve Myrcia sylvatica chemotypes. The occurrence of different chemical profiles can be attributed to the genetic variability of this species [9].

3. Materials and Methods

3.1. Plant Material

The leaves of the seven Myrcia sylvatica wild-growing specimens were collected on Caratateua Island, Belém, Pará state, Brazil, during the rainy season. The collection site, herbarium voucher number, and geographic coordinates are listed in Table 2. After identification, the plant specimens were deposited in the Herbarium of Museu Paraense Emílio Goeldi (MG) in the city of Belém, Brazil. The leaves were dried for three days at room temperature, ground, and then submitted to essential oil hydrodistillation in duplicate using a Clevenger-type apparatus. The oils obtained were dried over anhydrous sodium sulfate, and total oil yields were expressed as mL/100 g of the dried material [25,26]. The specimens were collected in agreement with the Brazilian laws concerning the protection of biodiversity (SISGEN A78F864).

3.2. Analysis of Essential Oil Composition

The oil composition analysis was performed by GC-MS, using a Shimadzu instrument Model QP-2010 ultra (Shimadzu, Tokyo, Japan) equipped with a Rtx-5MS (30 m × 0.25 mm; 0.25 μm film thickness) fused silica capillary column (Restek, Bellefonte, PA, USA). Helium was used as carrier gas, adjusted to 1.0 mL/min at 57.5 KPa; split injection (split ratio 1:20) of 1 μL of n-hexane solution (oil 5 μL: n-hexane 500 μL); injector and interface temperature were 250 °C; oven programmed temperature was 60 to 240 °C (3 °C/min), followed by an isotherm of 10 min. EIMS (electron impact mass spectrometry): electron energy, 70 eV; ion source temperature was 200 °C. The mass spectra were obtained by automatically scanning every 0.3 s, with mass fragments in the range of 35–400 m/z. The compounds present in the samples were identified by comparison of their mass spectrum and retention index, calculated for all volatile components using a linear equation by Van den Dool and Kratz [27], with the data present in the commercial libraries FFNSC-2 [16] and Adams [15]. The retention index was calculated using n-alkane standard solutions (C8–C40, Sigma-Aldrich, St. Louis, MO, USA) under the same chromatographic conditions. The GC-FID analysis was carried out on a Shimadzu QP-2010 instrument, equipped with an FID detector, in the same conditions, except that hydrogen was used as the carrier gas. The percentage composition of the oil samples was computed from the GC-FID peak areas. The analyses were carried out in triplicate.

3.3. Multivariate Statistical Analyses

The data matrix was standardized for the multivariate analysis by subtracting the mean and then dividing it by the standard deviation. The hierarchical grouping analysis (HCA), considering the Euclidean distance and complete linkage, was used to verify the similarity of the oil samples based on the distribution of the constituents selected. The principal component analysis (PCA) was applied to verify the interrelation among the oils’ components (>4%) (OriginPro trial version, OriginLab Corporation, Northampton, MA, USA).

4. Conclusions

The intraspecific chemical variability among the Myrcia sylvatica specimens studied was evidenced by the occurrence of four chemotypes, described here for the first time, with a predominance of the sesquiterpenes class in all samples. In addition to the chemotypes already described in the literature (8 chemotypes), it is possible that at least 10 Myrcia sylvatica chemotypes occur. Considering the potential of M. sylvatica, the knowledge of this variability can contribute to chemotaxonomy, economical use, and future studies that evaluate the biological properties of this species.

Author Contributions

Formal analysis, J.S.d.C., J.J.d.S.F., W.N.S., P.L.B.F., J.K.R.d.S. and J.G.S.M.; writing, proofreading and editing, P.L.B.F. and J.G.S.M.; conception, P.L.B.F. All authors have read and agreed to the published version of the manuscript.


This research was funded by the PAPQ (Programa de Apoio à Publicação Qualificada), Propesp, UFPa.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on request from the corresponding author.


The authors are grateful to the Fundação Amazônia de Amparo a Estudos e Pesquisas (FAPESPA, PA, Brazil) for providing scholarships to J.S.d.C. Additionally, we are grateful to the Aromatic Plant Research Center (APRC,, accessed on 25 March 2022).

Conflicts of Interest

The authors declare no conflict of interest.

Appendix A

Figure A1. Gas chromatography chromatogram chemotypes of Myrcia sylvatica.
Figure A1. Gas chromatography chromatogram chemotypes of Myrcia sylvatica.
Molecules 27 08975 g0a1


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Figure 1. Biosynthetic pathway of the main constituents from Myrcia sylvatica essential oil.
Figure 1. Biosynthetic pathway of the main constituents from Myrcia sylvatica essential oil.
Molecules 27 08975 g001
Figure 2. Dendrogram representing the similarity relation of the oil composition of Myrcia sylvatica.
Figure 2. Dendrogram representing the similarity relation of the oil composition of Myrcia sylvatica.
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Figure 3. Principal components analysis of the oils of Myrcia sylvatica.
Figure 3. Principal components analysis of the oils of Myrcia sylvatica.
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Table 1. Yield and composition of essential oils from Myrcia sylvatica leaves.
Table 1. Yield and composition of essential oils from Myrcia sylvatica leaves.
RI(C)RI(L)Constituents (%) *Msy-01Msy-02Msy-03Msy-04Msy-05Msy-06Msy-07
1847846 aHex-(2E)-enal0.1 tr0.10.5trtr
2850850 aHex-(3Z)-enol0.1tr0.
3858859 aHex-(2Z)-enol 0.10.2
4862863 an-Hexanol0.1
5904907 aButyl propanoate 0.1
6934932 aα-Pinene
7977974 aβ-Pinene 0.10.4tr
8991988 aMyrcene 0.10.7tr
910281024 aLimonene tr0.20.1tr
1011901186 aα-Terpineol 0.10.1
1111941190 aMethyl salicylate 0.1
1211951195 aMyrtenal 0.1
1313381335 aδ-Elemene0.4
1413491345 aα-Cubebene
1513521350 aCitronellyl acetate 0.1
1613681367 bCyclosativene 0.10.2 0.1
1713711373 aα-Ylangene
1813741374 aIsoledenetr 0.1
1913771374 aα-Copaene0.
2013811378 aHex-(3Z)-enyl hexenoate 0.1
2113861387 aβ-Bourbonene0.
2213901387 aβ-Cubebene 0.1
2313921389 aβ-Elemene1.
2414101409 aα-Gurjunene0.1 tr0.10.30.1
2514221417 aβ-Caryophyllene10.
2614281428 a(E)-α-Ionone
2714291430 aβ-Copaene0.20.10.3
2814291431 aβ-Gurjunene 0.9
2914341434 aγ-Elemene12. 7.62.9
3014371432 aα-trans-Bergamotene0.
3114401439 aAromadendrene0.
3214401437 aα-Guaiene 0.5
3314431442 aGuaia-6,9-diene 0.6
3414441445 bSelina-5,11-diene0.1
3514471448 acis-Muurola-3,5-dienetr 0.5
3614511447 aIsogermacrene D 0.20.2
3714511451 atrans-Muurola-3,5-diene0.1 0.5
3814541452 aα-Humulene1.
3914621460 aallo-Aromadendrene0.60.2
4014611464 a9-epi-(E)-Caryophyllene
4114631472 bcis-Cadina-1(6),4-diene 0.2
4214631465 acis-Muurola-4(14),5-diene 0.1
4314661471 aDauca-5,8-diene 0.1
4414741475 atrans-Cadina-1(6),4-diene0.2 0.2
4514771478 aγ-Muurolene0. 0.7
4614811483 aα-Amorphene 0.2
4714821484 aGermacrene D3.8
4814851476 bSelina-4,11-diene 0.7
4914871492 aβ-Selinene0.
5014911491 a10,11-epoxy-Calamenene 0.20.2
5114911493 atrans-Muurola-4(14),5-diene 0.5
5214921489 aδ-Selinene0.3
5314961496 aViridiflorene2.
5414971500 aBicyclogermacrene5.0 0.8
5515011500 aα-Muurolene0.
5615081502 atrans-β-Guaiene 1.0
5715081509 aα-Bulnesene 0.4 0.1
5815081511 aδ-Amorphene0.4 0.2
5915091505 aβ-Bisabolene 0.2 0.9
6015151513 aγ-Cadinene0.
6115191514 aCubebol 0.30.2
6215231521 atrans-Calamenene 0.6
6315241522 aδ-Cadinene1.8
6415311532 aγ-Cuprenene 0.1
6515331533 atrans-Cadina-1,4-diene0.1 tr0.1tr
6615361528 aZonarene 0.3 0.20.2
6715391537 aα-Cadinene 0.2 0.70.3
6815391540 bSelina-4(15),7(11)-diene1.6 0.6 1.50.7
6915431545 aSelina-3,7(11)-diene1.90.2 1.50.6
7015441544 aα-Calacorene
7115581559 aGermacrene B24.
7215581562 aepi-Longipinanol 0.3
7315631564 aβ-Calacorene
7415681567 aPalustrol0.83.3 1.8
7515781577 aSpathulenol2.913.411.19.810.115.716.0
7615841582 aCaryophyllene oxide3.315.
7715921592 aViridiflorol1.
7815831590 aGlobulol
7915951595 aCubeban-11-ol0.72.9
8016021600 aRosifoliol0.
8116091608 aHumulene epoxide
8216151618 a1,10-di-epi-Cubenol
8316181618 aJunenol
8416291627 a1-epi-Cubenol 1.8
8516291630 aMuurola-4,10(14)-dien-1β-ol 5.8
8616291632 aα-Acorenol0.8
8716331630 aγ-Eudesmol0.3 0.61.0
8816371639 aCaryophylla-4(12),8(13)-dien-5β-ol 0.7 0.6
8916431645 aCubenol 0.30.7
9016431640 aepi-α-Murrolol 4.83.2
9116431640 bepi-α-Cadinol1.61.4 5.11.2
9216471644 aα-Muurolol0.
9316491648 acis-Guaia-3,9-dien-11-ol 0.9
9416551652 aα-Cadinol1.
9516661668 bIntermedeol0.90.70.8 0.81.0
9616681668 atrans-Calamenen-10-ol 0.10.1
9716711668 a14-hydroxy-9-epi-(E)-Caryophyllene 1.9
9816751675 aCadalene
9916771676 aMustakone 0.7
10016851679 aKusinol 0.7
10116861664 aLongiborneol acetate0.1
10216901685 aGermacra-4(15),5,10(14)-trien-1α-ol 0.2 0.10.2
10316961696 bJuniper camphor1. 2.13.3
10417011702 a10-nor-Calamenen-10-one 0.2
10517391733 aIsobicyclogermacrenal 0.2 0.1
10617621766 aDrimenol 0.2
10717711767 a14-oxy-α-Muurolene 0.2
10817801779 a14-hydroxy-α-Muurolene 0.10.1tr 0.2
10917981792 aβ-Eudesmol acetate 0.2
11018011803 a14-hydroxy-δ-Cadinene tr0.1 0.1
11118361845 a(2E,6E)-Farnesyl acetate 0.1
11221132106 bPhytol0.1 0.10.3
Monoterpene hydrocarbons---
Oxygenated monoterpenoids--tr0.30.10.1-
Sesquiterpene hydrocarbons71.812.533.143.547.748.029.0
Oxygenated sesquiterpenoids17.471.539.736.036.729.940.4
Oil yield (%) *
RI(C) = calculated retention index using an n-alkane standard solution (C8–C40) in Rtx-5MS column; RI(L) = literature retention index. * Main constituents in bold, n = 2 (standard deviation was less than 2.0% in chemical composition and <0.1% in oil yield); tr = traces (% < 0.1); Msy = Myrcia sylvatica; a = Adams library [15]; b = FFNCS library [16].
Table 2. Collection site, herbarium voucher number, and geographic coordinates for the Myrcia sylvatica specimens.
Table 2. Collection site, herbarium voucher number, and geographic coordinates for the Myrcia sylvatica specimens.
CodeVoucher NumberCoordinates Latitude/Longitude
Msyl-1MG-2287381°15′52.65″S, 48°28′12.85″W
Msyl-2MG-2292171°14′52.69″S, 48°26′30.20″W
Msyl-3MG-2299551°15′52.42″S, 48°28′12.58″W
Msyl-4MG-2299561°15′52.41″S, 48°28′12.69″W
Msyl-5MG-2299541°15′42.54″S, 48°28′1.78″W
Msyl-6MG-2332831°14′51.71″S, 48°26′29.66″W
Msyl-7MG-2332841°14′20.79″S, 48°26′9.94″W
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MDPI and ACS Style

Costa, J.S.d.; Freitas, J.J.d.S.; Setzer, W.N.; da Silva, J.K.R.; Maia, J.G.S.; Figueiredo, P.L.B. Variability in the Chemical Composition of Myrcia sylvatica (G. Mey) DC. Essential Oils Growing in the Brazilian Amazon. Molecules 2022, 27, 8975.

AMA Style

Costa JSd, Freitas JJdS, Setzer WN, da Silva JKR, Maia JGS, Figueiredo PLB. Variability in the Chemical Composition of Myrcia sylvatica (G. Mey) DC. Essential Oils Growing in the Brazilian Amazon. Molecules. 2022; 27(24):8975.

Chicago/Turabian Style

Costa, Jamile Silva da, Jofre Jacob da Silva Freitas, William N. Setzer, Joyce Kelly R. da Silva, José Guilherme S. Maia, and Pablo Luis B. Figueiredo. 2022. "Variability in the Chemical Composition of Myrcia sylvatica (G. Mey) DC. Essential Oils Growing in the Brazilian Amazon" Molecules 27, no. 24: 8975.

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