Rosmarinic Acid Prevents Cisplatin-Induced Liver and Kidney Injury by Inhibiting Inflammatory Responses and Enhancing Total Antioxidant Capacity, Thereby Activating the Nrf2 Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Effects of RA on Body Weight Changes and Organ Indices in Mice with CP-ALI and AKI
2.2. Effects of RA on Liver and Kidney Function in Mice with CP-ALI and AKI
2.3. Effect of RA on Serum Levels of IL-1β, IL-6 and TNF-α Induced by CP
2.4. Effects of RA on the Antioxidant Capacity of the Liver and Kidney in the Presence of CP
2.5. Effects of RA on mRNA Expression in CP-Induced ALI and AKI
2.6. Effects of RA on Histological Changes of CP-Induced ALI and AKI in Mice
2.7. Effects of RA on CP-Induced mRNA Expression Levels of the Nrf2 Signaling Pathway
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Reagents
4.3. Challenge and Treatment of Mice
4.4. Collection and Testing of Blood and Organs
4.5. Real-Time Polymerase Chain Reaction (qPCR)
4.6. Liver and Kidney Histology
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Targets | Forward | Reverse |
---|---|---|
IL-1β | AAAAAAGCCTCGTGCTGTCG | GTCGTTGCTTGGTTCTCCTTG |
IL-6 | CTAGTGCGTTATGCCTAAGC | ATAGTGTCCCAACATTCATATTGTC |
TNF-α | AGCACAGAAAGCATGATCCG | CTGATGAGAGGGAGGCCATT |
SOD | CGGCTTCTCGTCTTGCT | GTTCACCGCTTGCCTTC |
CAT | GCCAACTACCAGCGTGA | CCGCACCTGAGTGACAT |
GPx | GGCATTGGCTTGGTGATT | AGGTGGAAAGGCATCGG |
Nrf2 | GCTCCTATGCGTGAATCCCA | TTCTGGGCGGCGACTTTATT |
HO-1 | GTCAAGCACAGGGTGACAGA | AAGTGACGCCATCTGTGAGG |
Keap1 | ACCTCGGACTCGCAGCGTAC | CCAAGCAGGAGGAGTTCTTCAACC |
GCLC | CTGCACATCTACCACGCAGT | GTCTCAAGAACATCGCCTCC |
GCLM | CGGGAACCTGCTCAACTG | CCAAAACATCTGGAAACTCCC |
β-actin | ATCACTATTGGCAACGAGCG | TCAGCAATGCCTGGGTACAT |
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Xiang, Y.; Ji, M.; Wu, L.; Lv, L.; Liang, Q.; Deng, R.; Deng, Z.; Liu, X.; Ren, L.; Feng, X.; et al. Rosmarinic Acid Prevents Cisplatin-Induced Liver and Kidney Injury by Inhibiting Inflammatory Responses and Enhancing Total Antioxidant Capacity, Thereby Activating the Nrf2 Signaling Pathway. Molecules 2022, 27, 7815. https://doi.org/10.3390/molecules27227815
Xiang Y, Ji M, Wu L, Lv L, Liang Q, Deng R, Deng Z, Liu X, Ren L, Feng X, et al. Rosmarinic Acid Prevents Cisplatin-Induced Liver and Kidney Injury by Inhibiting Inflammatory Responses and Enhancing Total Antioxidant Capacity, Thereby Activating the Nrf2 Signaling Pathway. Molecules. 2022; 27(22):7815. https://doi.org/10.3390/molecules27227815
Chicago/Turabian StyleXiang, Yifei, Min Ji, Liqin Wu, Li Lv, Qiuling Liang, Ruihan Deng, Zhaoyou Deng, Xia Liu, Lingyi Ren, Xin Feng, and et al. 2022. "Rosmarinic Acid Prevents Cisplatin-Induced Liver and Kidney Injury by Inhibiting Inflammatory Responses and Enhancing Total Antioxidant Capacity, Thereby Activating the Nrf2 Signaling Pathway" Molecules 27, no. 22: 7815. https://doi.org/10.3390/molecules27227815
APA StyleXiang, Y., Ji, M., Wu, L., Lv, L., Liang, Q., Deng, R., Deng, Z., Liu, X., Ren, L., Feng, X., & He, J. (2022). Rosmarinic Acid Prevents Cisplatin-Induced Liver and Kidney Injury by Inhibiting Inflammatory Responses and Enhancing Total Antioxidant Capacity, Thereby Activating the Nrf2 Signaling Pathway. Molecules, 27(22), 7815. https://doi.org/10.3390/molecules27227815