Improvement of an Automated Sample Injection System for Pillar Array Columns to Increase Analytical Reproducibility
Abstract
:1. Introduction
2. Results and Discussion
2.1. Previously Developed Automated Sample Injection System
2.2. Improved Automated Sample Injection System
2.3. Optimization of the Improved Automated Sample Injection System
2.4. Evaluation of the Improved Automated Sample Injection System
2.5. Application of the System to Analyze Amino Acids in Human Plasma
3. Conclusions
4. Materials and Methods
4.1. Chemicals
4.2. Microchip Fabrication and Modification
4.3. Derivatization Conditions for Amino Acids with NBD-F
4.4. Pretreatment of Human Plasma
4.5. Chromatographic Conditions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
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Kuroki, H.; Koyama, H.; Tsunoda, M. Improvement of an Automated Sample Injection System for Pillar Array Columns to Increase Analytical Reproducibility. Molecules 2022, 27, 4715. https://doi.org/10.3390/molecules27154715
Kuroki H, Koyama H, Tsunoda M. Improvement of an Automated Sample Injection System for Pillar Array Columns to Increase Analytical Reproducibility. Molecules. 2022; 27(15):4715. https://doi.org/10.3390/molecules27154715
Chicago/Turabian StyleKuroki, Hiroshi, Hirotaka Koyama, and Makoto Tsunoda. 2022. "Improvement of an Automated Sample Injection System for Pillar Array Columns to Increase Analytical Reproducibility" Molecules 27, no. 15: 4715. https://doi.org/10.3390/molecules27154715