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Article

Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria

1
Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan
2
Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University (TWIns), 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Miguel Palma Lovillo, Ceferino A. Carrera Fernández and Ana Ruiz Rodríguez
Molecules 2022, 27(1), 256; https://doi.org/10.3390/molecules27010256
Received: 10 December 2021 / Revised: 28 December 2021 / Accepted: 30 December 2021 / Published: 31 December 2021
We have developed a convenient and selective method for the detection of Gram-positive bacteria using a ditopic poly(amidoamine) (PAMAM) dendrimer probe. The dendrimer that was modified with dipicolylamine (dpa) and phenylboronic acid groups showed selectivity toward Staphylococcus aureus. The ditopic dendrimer system had higher sensitivity and better pH tolerance than the monotopic PAMAM dendrimer probe. We also investigated the mechanisms of various ditopic PAMAM dendrimer probes and found that the selectivity toward Gram-positive bacteria was dependent on a variety of interactions. Supramolecular interactions, such as electrostatic interaction and hydrophobic interaction, per se, did not contribute to the bacterial recognition ability, nor did they improve the selectivity of the ditopic dendrimer system. In contrast, the ditopic PAMAM dendrimer probe that had a phosphate-sensing dpa group and formed a chelate with metal ions showed improved selectivity toward S. aureus. The results suggested that the targeted ditopic PAMAM dendrimer probe showed selectivity toward Gram-positive bacteria. This study is expected to contribute to the elucidation of the interaction between synthetic molecules and bacterial surface. Moreover, our novel method showed potential for the rapid and species-specific recognition of various bacteria. View Full-Text
Keywords: bacterial recognition; dendrimer; dipicolylamine; E. coli; phenylboronic acid; S. aureus bacterial recognition; dendrimer; dipicolylamine; E. coli; phenylboronic acid; S. aureus
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MDPI and ACS Style

Mikagi, A.; Manita, K.; Yoyasu, A.; Tsuchido, Y.; Kanzawa, N.; Hashimoto, T.; Hayashita, T. Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria. Molecules 2022, 27, 256. https://doi.org/10.3390/molecules27010256

AMA Style

Mikagi A, Manita K, Yoyasu A, Tsuchido Y, Kanzawa N, Hashimoto T, Hayashita T. Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria. Molecules. 2022; 27(1):256. https://doi.org/10.3390/molecules27010256

Chicago/Turabian Style

Mikagi, Ayame, Koichi Manita, Asuka Yoyasu, Yuji Tsuchido, Nobuyuki Kanzawa, Takeshi Hashimoto, and Takashi Hayashita. 2022. "Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria" Molecules 27, no. 1: 256. https://doi.org/10.3390/molecules27010256

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