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Article

Bioactive Compounds Obtained from Polish “Marynka” Hop Variety Using Efficient Two-Step Supercritical Fluid Extraction and Comparison of Their Antibacterial, Cytotoxic, and Anti-Proliferative Activities In Vitro

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Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland
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Supercritical Extraction Department, Łukasiewicz Research Network- New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13a, 24-110 Puławy, Poland
*
Authors to whom correspondence should be addressed.
Academic Editors: Hosam O. Elansary and Agnieszka Szopa
Molecules 2021, 26(8), 2366; https://doi.org/10.3390/molecules26082366
Received: 19 March 2021 / Revised: 15 April 2021 / Accepted: 17 April 2021 / Published: 19 April 2021
Given the health-beneficial properties of compounds from hop, there is still a growing trend towards developing successful extraction methods with the highest yield and also receiving the products with high added value. The aim of this study was to develop efficient extraction method for isolation of bioactive compounds from the Polish “Marynka” hop variety. The modified two-step supercritical fluid extraction allowed to obtain two hop samples, namely crude extract (E1), composed of α-acids, β-acids, and terpene derivatives, as well as pure xanthohumol with higher yield than that of other available methods. The post-extraction residues (R1) were re-extracted in order to obtain extract E2 enriched in xanthohumol. Then, both samples were subjected to investigation of their antibacterial (anti-acne, anti-caries), cytotoxic, and anti-proliferative activities in vitro. It was demonstrated that extract (E1) possessed more beneficial biological properties than xanthohumol. It exhibited not only better antibacterial activity against Gram-positive bacteria strains (MIC, MBC) but also possessed a higher synergistic effect with commercial antibiotics when compared to xanthohumol. Moreover, cell culture experiments revealed that crude extract neither inhibited viability nor divisions of normal skin fibroblasts as strongly as xanthohumol. In turn, calculated selectivity indexes showed that the crude extract had from slightly to significantly better selective anti-proliferative activity towards cancer cells in comparison with xanthohumol. View Full-Text
Keywords: hop; Humulus lupulus L.; supercritical extraction; Marynka strain; xanthohumol; antibacterial activity; cytotoxicity; anti-proliferative activity hop; Humulus lupulus L.; supercritical extraction; Marynka strain; xanthohumol; antibacterial activity; cytotoxicity; anti-proliferative activity
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MDPI and ACS Style

Klimek, K.; Tyśkiewicz, K.; Miazga-Karska, M.; Dębczak, A.; Rój, E.; Ginalska, G. Bioactive Compounds Obtained from Polish “Marynka” Hop Variety Using Efficient Two-Step Supercritical Fluid Extraction and Comparison of Their Antibacterial, Cytotoxic, and Anti-Proliferative Activities In Vitro. Molecules 2021, 26, 2366. https://doi.org/10.3390/molecules26082366

AMA Style

Klimek K, Tyśkiewicz K, Miazga-Karska M, Dębczak A, Rój E, Ginalska G. Bioactive Compounds Obtained from Polish “Marynka” Hop Variety Using Efficient Two-Step Supercritical Fluid Extraction and Comparison of Their Antibacterial, Cytotoxic, and Anti-Proliferative Activities In Vitro. Molecules. 2021; 26(8):2366. https://doi.org/10.3390/molecules26082366

Chicago/Turabian Style

Klimek, Katarzyna, Katarzyna Tyśkiewicz, Malgorzata Miazga-Karska, Agnieszka Dębczak, Edward Rój, and Grazyna Ginalska. 2021. "Bioactive Compounds Obtained from Polish “Marynka” Hop Variety Using Efficient Two-Step Supercritical Fluid Extraction and Comparison of Their Antibacterial, Cytotoxic, and Anti-Proliferative Activities In Vitro" Molecules 26, no. 8: 2366. https://doi.org/10.3390/molecules26082366

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