Anti-Inflammatory Properties In Vitro and Hypoglycaemic Effects of Phenolics from Cultivated Fruit Body of Phellinus baumii in Type 2 Diabetic Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of PPE
2.2. Measurement of Anti-Inflammatory Activity In Vitro
2.3. Animal Breeding Conditions and Treatments
2.4. Determination of Serum and Tissue Biochemical Index in Mice
2.5. Oral Glucose Tolerance Test
2.6. Quantitative Real-Time PCR
2.7. Hematoxylin and Eosin Staining
2.8. Statistical Analysis
3. Results and Discussion
3.1. Anti-Inflammatory Properties of PPE
3.2. Effect of PPE on the Body Weight Gain and Blood Glucose Level
3.3. Dynamic Changes of Blood Glucose during PPE Treatment in Mice
3.4. Effects of PPE on OGTT in Mice
3.5. Effects of PPE on Insulin and Insulin Resistance Index in Mice
3.6. Effects of PPE on Serum Glucose Metabolism in Mice
3.7. Effects of PPE on Serum Lipid Metabolism in Mice
3.8. Effects of PPE on Gene Expression in the IRS1/PI3K/AKT Pathway in Mouse Liver
3.9. Effects of PPE on the Histomorphology of Mouse Liver
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Group | Body Weight of Mice (g) | ||||||
---|---|---|---|---|---|---|---|
0 d | 10 d | 20 d | 30 d | 40 d | 50 d | 60 d | |
NC | 25.2 ± 1.1 | 26.4 ± 1.0 | 28.7 ± 1.1 | 31.1 ± 1.5 | 33.0 ± 1.5 | 34.9 ± 1.2 | 35.9 ± 1.5 |
MC | 28.2 ± 1.0 ## | 29.7 ± 1.2 ## | 32.4 ± 1.3 ## | 35.0 ± 1.4 ## | 38.3 ± 1.5 ## | 40.4 ± 1.6 ## | 42.1 ± 2.2 |
Met | 28.4 ± 1.1 | 29.6 ± 1.2 | 32.3 ± 1.6 | 34.1 ± 1.7 | 36.2 ± 1.7 * | 38.4 ± 1.9 * | 39.8 ± 2.3 * |
L | 27.7 ± 1.6 | 28.7 ± 1.7 | 31.5 ± 1.8 | 33.6 ± 1.9 | 36.1 ± 2.2 ** | 38.7 ± 2.7 * | 40.2 ± 3.1 |
M | 28.3 ± 1.5 | 29.4 ± 1.8 | 32.4 ± 2.1 | 34.7 ± 2.2 | 36.8 ± 2.2 | 38.9 ± 2.1 | 40.4 ± 2.2 |
H | 27.9 ± 1.5 | 28.9 ± 1.5 | 31.7 ± 2.0 | 33.9 ± 1.9 | 36.1 ± 2.0 ** | 38.2 ± 2.1 * | 39.3 ± 2.1 ** |
Group | Body Weight Gain of Mice (g) | ||||||
NC | 0 | 1.2 ± 0.6 | 3.5 ± 1.3 | 5.9 ± 1.8 | 7.8 ± 1.7 | 9.6 ± 1.54 | 10.7 ± 1.6 |
MC | 0 | 1.5 ± 0.4 | 4.3 ± 0.8 | 6.8 ± 0.7 | 10.1 ± 0.8 ## | 12.3 ± 1.1 ## | 13.9 ± 1.6 ## |
Met | 0 | 1.2 ± 0.3 | 3.9 ± 0.8 | 5.7 ± 0.8 * | 7.7 ± 0.9 ** | 10.0 ± 1.3 ** | 11.4 ± 1.6 ** |
L | 0 | 0.9 ± 0.3 ** | 3.7 ± 0.8 | 5.9 ± 0.8 | 8.3 ± 1.1 ** | 11.0 ± 1.58 * | 12.5 ± 1.9 * |
M | 0 | 1.1 ± 0.5 * | 4.0 ± 0.7 | 6.4 ± 1.0 | 8.5 ± 1.0 ** | 10.5 ± 1.3 ** | 12.0 ± 1.1 ** |
H | 0 | 1.0 ± 0.6 * | 3.7 ± 1.0 | 6.0 ± 1.4 | 8.1 ± 1.7 ** | 10.2 ± 1.9 ** | 11.4 ± 2.1 ** |
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Yang, K.; Zhang, S.; Geng, Y.; Tian, B.; Cai, M.; Guan, R.; Li, Y.; Ye, B.; Sun, P. Anti-Inflammatory Properties In Vitro and Hypoglycaemic Effects of Phenolics from Cultivated Fruit Body of Phellinus baumii in Type 2 Diabetic Mice. Molecules 2021, 26, 2285. https://doi.org/10.3390/molecules26082285
Yang K, Zhang S, Geng Y, Tian B, Cai M, Guan R, Li Y, Ye B, Sun P. Anti-Inflammatory Properties In Vitro and Hypoglycaemic Effects of Phenolics from Cultivated Fruit Body of Phellinus baumii in Type 2 Diabetic Mice. Molecules. 2021; 26(8):2285. https://doi.org/10.3390/molecules26082285
Chicago/Turabian StyleYang, Kai, Su Zhang, Yan Geng, Baoming Tian, Ming Cai, Rongfa Guan, Yougui Li, Bangwei Ye, and Peilong Sun. 2021. "Anti-Inflammatory Properties In Vitro and Hypoglycaemic Effects of Phenolics from Cultivated Fruit Body of Phellinus baumii in Type 2 Diabetic Mice" Molecules 26, no. 8: 2285. https://doi.org/10.3390/molecules26082285