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Determination of Intact Parabens in the Human Plasma of Cancer and Non-Cancer Patients Using a Validated Fabric Phase Sorptive Extraction Reversed-Phase Liquid Chromatography Method with UV Detection

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Laboratory of Pharmaceutical Analysis, Division of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece
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Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA
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Department for Plastic, Reconstructive and Aesthetic Surgery, University Hospital Dubrava, 10 000 Zagreb, Croatia
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Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
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Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10 000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: Farid Chemat
Molecules 2021, 26(6), 1526; https://doi.org/10.3390/molecules26061526
Received: 31 January 2021 / Revised: 7 March 2021 / Accepted: 8 March 2021 / Published: 11 March 2021
Parabens have been widely employed as preservatives since the 1920s for extending the shelf life of foodstuffs, medicines, and daily care products. Given the fact that there are some legitimate concerns related to their potential multiple endocrine-disrupting properties, the development of novel bioanalytical methods for their biomonitoring is crucial. In this study, a fabric phase sorptive extraction reversed-phase liquid chromatography method coupled with UV detection (FPSE-HPLC-UV) was developed and validated for the quantitation of seven parabens in human plasma samples. Chromatographic separation of the seven parabens and p-hydroxybenzoic acid was achieved on a semi-micro Spherisorb ODS1 analytical column under isocratic elution using a mobile phase containing 0.1% (v/v) formic acid and 66% 49 mM ammonium formate aqueous solution in acetonitrile at flow rate 0.25 mL min−1 with a 24-min run time for each sample. The method was linear at a concentration range of 20 to 500 ng mL−1 for the seven parabens under study in human plasma samples. The efficiency of the method was proven with the analysis of 20 human plasma samples collected from women subjected to breast cancer surgery and to reconstructive and aesthetic breast surgery. The highest quantitation rates in human plasma samples from cancerous cases were found for methylparaben and isobutylparaben with average plasma concentrations at 77 and 112.5 ng mL−1. The high concentration levels detected agree with previous findings for some of the parabens and emphasize the need for further epidemiological research on the possible health effects of the use of these compounds. View Full-Text
Keywords: fabric phase sorptive extraction (FPSE); parabens; p-hydroxybenzoic acid esters; human plasma; bioanalysis fabric phase sorptive extraction (FPSE); parabens; p-hydroxybenzoic acid esters; human plasma; bioanalysis
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MDPI and ACS Style

Parla, A.; Zormpa, E.; Paloumpis, N.; Kabir, A.; Furton, K.G.; Roje, Ž.; Samanidou, V.; Vinković Vrček, I.; Panderi, I. Determination of Intact Parabens in the Human Plasma of Cancer and Non-Cancer Patients Using a Validated Fabric Phase Sorptive Extraction Reversed-Phase Liquid Chromatography Method with UV Detection. Molecules 2021, 26, 1526. https://doi.org/10.3390/molecules26061526

AMA Style

Parla A, Zormpa E, Paloumpis N, Kabir A, Furton KG, Roje Ž, Samanidou V, Vinković Vrček I, Panderi I. Determination of Intact Parabens in the Human Plasma of Cancer and Non-Cancer Patients Using a Validated Fabric Phase Sorptive Extraction Reversed-Phase Liquid Chromatography Method with UV Detection. Molecules. 2021; 26(6):1526. https://doi.org/10.3390/molecules26061526

Chicago/Turabian Style

Parla, Anthi, Eirini Zormpa, Nikolaos Paloumpis, Abuzar Kabir, Kenneth G. Furton, Željka Roje, Victoria Samanidou, Ivana Vinković Vrček, and Irene Panderi. 2021. "Determination of Intact Parabens in the Human Plasma of Cancer and Non-Cancer Patients Using a Validated Fabric Phase Sorptive Extraction Reversed-Phase Liquid Chromatography Method with UV Detection" Molecules 26, no. 6: 1526. https://doi.org/10.3390/molecules26061526

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