Next Article in Journal
Structure-Based Virtual Screening Identifies Multiple Stable Binding Sites at the RecA Domains of SARS-CoV-2 Helicase Enzyme
Next Article in Special Issue
The Common Cichory (Cichorium intybus L.) as a Source of Extracts with Health-Promoting Properties—A Review
Previous Article in Journal
Spectroscopic Study of the Molecular Structure of the New Hybrid with a Potential Two-Way Antibacterial Effect
Previous Article in Special Issue
In Vitro Phytotherapeutic Properties of Aqueous Extracted Adenia viridiflora Craib. towards Civilization Diseases
Article

Extract from the Marine Seaweed Padina pavonica Protects Mitochondrial Biomembranes from Damage by Amyloidogenic Peptides

1
Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, 2023 Msida, Malta
2
Centre for Molecular Medicine and Biobanking, Biomedical Sciences Building, University of Malta, 2023 Msida, Malta
3
Department of Experimental Biology, Faculty of Science, Masaryk University, 60300 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Raffaele Capasso
Molecules 2021, 26(5), 1444; https://doi.org/10.3390/molecules26051444
Received: 5 February 2021 / Revised: 2 March 2021 / Accepted: 4 March 2021 / Published: 7 March 2021
The identification of compounds which protect the double-membrane of mitochondrial organelles from disruption by toxic confomers of amyloid proteins may offer a therapeutic strategy to combat human neurodegenerative diseases. Here, we exploited an extract from the marine brown seaweed Padina pavonica (PPE) as a vital source of natural bioactive compounds to protect mitochondrial membranes against insult by oligomeric aggregates of the amyloidogenic proteins amyloid-β (Aβ), α-synuclein (α-syn) and tau, which are currently considered to be major targets for drug discovery in Alzheimer’s disease (AD) and Parkinson’s disease (PD). We show that PPE manifested a significant inhibitory effect against swelling of isolated mitochondria exposed to the amyloid oligomers, and attenuated the release of cytochrome c from the mitochondria. Using cardiolipin-enriched synthetic lipid membranes, we also show that dye leakage from fluorophore-loaded vesicles and formation of channel-like pores in planar bilayer membranes are largely prevented by incubating the oligomeric aggregates with PPE. Lastly, we demonstrate that PPE curtails the ability of Aβ42 and α-syn monomers to self-assemble into larger β-aggregate structures, as well as potently disrupts their respective amyloid fibrils. In conclusion, the mito-protective and anti-aggregator biological activities of Padina pavonica extract may be of therapeutic value in neurodegenerative proteinopathies, such as AD and PD. View Full-Text
Keywords: Padina pavonica seaweed extract; Alzheimer’s disease; Parkinson’s disease; mitochondria; amyloidogenic proteins; membrane permeabilization Padina pavonica seaweed extract; Alzheimer’s disease; Parkinson’s disease; mitochondria; amyloidogenic proteins; membrane permeabilization
Show Figures

Figure 1

MDPI and ACS Style

Caruana, M.; Camilleri, A.; Farrugia, M.Y.; Ghio, S.; Jakubíčková, M.; Cauchi, R.J.; Vassallo, N. Extract from the Marine Seaweed Padina pavonica Protects Mitochondrial Biomembranes from Damage by Amyloidogenic Peptides. Molecules 2021, 26, 1444. https://doi.org/10.3390/molecules26051444

AMA Style

Caruana M, Camilleri A, Farrugia MY, Ghio S, Jakubíčková M, Cauchi RJ, Vassallo N. Extract from the Marine Seaweed Padina pavonica Protects Mitochondrial Biomembranes from Damage by Amyloidogenic Peptides. Molecules. 2021; 26(5):1444. https://doi.org/10.3390/molecules26051444

Chicago/Turabian Style

Caruana, Mario, Angelique Camilleri, Maria Y. Farrugia, Stephanie Ghio, Michaela Jakubíčková, Ruben J. Cauchi, and Neville Vassallo. 2021. "Extract from the Marine Seaweed Padina pavonica Protects Mitochondrial Biomembranes from Damage by Amyloidogenic Peptides" Molecules 26, no. 5: 1444. https://doi.org/10.3390/molecules26051444

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop