Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis
Abstract
:1. Introduction
2. Results
2.1. Effects of Baicalin on the Expression of Tight Junctions Genes in Peritoneum of G. parasuis Challenged Piglets
2.2. Effect of Baicalin on the Distibution Patterns of Tight Junctions in Peritoneum of G. parasuis Challenged Piglets
2.3. Effect of Baicalin on PKC and MLCK/MLC Signaling Pathways in Peritoneum of G. parasuis Infected Piglets
2.4. Histopathological Analysis
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains
4.2. Experimental Products
4.3. Experimental Animals, Management, and Design
4.4. Experimental Sample Collection
4.5. RNA Extraction and RT-PCR
4.6. Immunofluorescence Microscopy
4.7. Western Blotting Analysis
4.8. Histopathology
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Gene | Nucleotide Sequences (5’–3’) | Tm (°C) | Length (bp) | |
---|---|---|---|---|
β-actin | Forward | TGCGGGACATCAAGGAGAAG | 57.4 | 216 |
Reverse | AGTTGAAGGTGGTCTCGTGG | 57.4 | ||
Claudin-1 | Forward | CCTTGCTGAATCTGAACAC | 49.5 | 135 |
Reverse | GCACCTCATCATCTTCCAT | 50.0 | ||
JAM-1 | Forward | TGACAGAACAGGCGAATG | 50.1 | 167 |
Reverse | GCAGCATAGGCAGGAATT | 50.1 | ||
ZO-1 | Forward | GAAGATGATGAAGATGAGGATG | 50.3 | 184 |
Reverse | GGAGGATGCTGTTGTCTC | 49.9 | ||
Occludin | Forward | GAGTGATTCGGATTCTGTCT | 50.3 | 181 |
Reverse | TAGCCATAACCATAGCCATAG | 50.2 |
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Zhang, J.; Zhang, Z.; Xu, J.; Ye, C.; Fu, S.; Hu, C.-A.A.; Qiu, Y.; Liu, Y. Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis. Molecules 2021, 26, 1268. https://doi.org/10.3390/molecules26051268
Zhang J, Zhang Z, Xu J, Ye C, Fu S, Hu C-AA, Qiu Y, Liu Y. Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis. Molecules. 2021; 26(5):1268. https://doi.org/10.3390/molecules26051268
Chicago/Turabian StyleZhang, Jiacheng, Zhaoran Zhang, Jianfeng Xu, Chun Ye, Shulin Fu, Chien-An Andy Hu, Yinsheng Qiu, and Yu Liu. 2021. "Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis" Molecules 26, no. 5: 1268. https://doi.org/10.3390/molecules26051268