Effects of Probiotic Culture Supernatant on Cariogenic Biofilm Formation and RANKL-Induced Osteoclastogenesis in RAW 264.7 Macrophages
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of Probiotic Strains on the Growth of S. mutans
2.2. Effect of SCSs on S. mutans-Induced Preformed Biofilm Formation
2.3. Effect of SCSs on RANKL-Induced Osteoclast Differentiation
2.4. Effect of the SCS of L. salivarius MG4265 on Osteoclast Specific Transcriptional Factors and Osteoclast-Associated Gene Expression
2.5. Effect of the SCS of L. salivarius MG4265 on RANKL-Induced Mitogen Activated Protein Kinase (MAPKs) Activation
2.6. Effect of the SCS of L. salivarius MG4265 on HO-1 Induction
3. Materials and Methods
3.1. Strains and Cultivation
3.2. Preparation of SCSs
3.3. Effect of Probiotic Strains on the Growth of S. mutans
3.4. Effect of SCSs on the Growth of S. mutans
3.5. Effect of SCSs on S. mutans-Induced Biofilm Formation
3.6. RANKL-Induced Osteoclast Differentiation of Murine Osteoclast Progenitor RAW 264.7 Cells
3.7. Tartrate-Resistant Acid Phosphatase-Positive (TRAP) Activity
3.8. RNA Extraction and Quantitative Real Time PCR (qRT-PCR)
3.9. Western Blotting Analysis
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Jung, J.-I.; Baek, S.-M.; Nguyen, T.H.; Kim, J.W.; Kang, C.-H.; Kim, S.; Imm, J.-Y. Effects of Probiotic Culture Supernatant on Cariogenic Biofilm Formation and RANKL-Induced Osteoclastogenesis in RAW 264.7 Macrophages. Molecules 2021, 26, 733. https://doi.org/10.3390/molecules26030733
Jung J-I, Baek S-M, Nguyen TH, Kim JW, Kang C-H, Kim S, Imm J-Y. Effects of Probiotic Culture Supernatant on Cariogenic Biofilm Formation and RANKL-Induced Osteoclastogenesis in RAW 264.7 Macrophages. Molecules. 2021; 26(3):733. https://doi.org/10.3390/molecules26030733
Chicago/Turabian StyleJung, Jae-In, Seung-Min Baek, Trung Hau Nguyen, Jin Woo Kim, Chang-Ho Kang, Seonyoung Kim, and Jee-Young Imm. 2021. "Effects of Probiotic Culture Supernatant on Cariogenic Biofilm Formation and RANKL-Induced Osteoclastogenesis in RAW 264.7 Macrophages" Molecules 26, no. 3: 733. https://doi.org/10.3390/molecules26030733