Next Article in Journal
A Comparative Analysis of Conventional and Deep Eutectic Solvent (DES)-Mediated Strategies for the Extraction of Chitin from Marine Crustacean Shells
Next Article in Special Issue
The Effect of Dia2 Protein Deficiency on the Cell Cycle, Cell Size, and Recruitment of Ctf4 Protein in Saccharomyces cerevisiae
Previous Article in Journal
Columnar Aggregates of Azobenzene Stars: Exploring Intermolecular Interactions, Structure, and Stability in Atomistic Simulations
Previous Article in Special Issue
The Influence of 5′,8-Cyclo-2′-deoxypurines on the Mitochondrial Repair of Clustered DNA Damage in Xrs5 Cells: The Preliminary Study
 
 
Article

In Silico Investigation of the Biological Implications of Complex DNA Damage with Emphasis in Cancer Radiotherapy through a Systems Biology Approach

1
Izmir Biomedicine and Genome Center, Balcova, Izmir 35340, Turkey
2
Izmir International Biomedicine and Genome Institute, Genomics and Molecular Biotechnology Department, Dokuz Eylül University, Balcova, Izmir 35220, Turkey
3
Physics Department, School of Applied Mathematical and Physical Sciences, National Technical University of Athens (NTUA), 15780 Zografou, Greece
4
IRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM U1194, Université de Montpellier, Institut Régional du Cancer de Montpellier, 34298 Montpellier, France
5
Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Boleslaw Karwowski and Jussara Amato
Molecules 2021, 26(24), 7602; https://doi.org/10.3390/molecules26247602
Received: 4 November 2021 / Revised: 7 December 2021 / Accepted: 11 December 2021 / Published: 15 December 2021
(This article belongs to the Special Issue The Beauty of Clustered DNA Lesion)
Different types of DNA lesions forming in close vicinity, create clusters of damaged sites termed as “clustered/complex DNA damage” and they are considered to be a major challenge for DNA repair mechanisms resulting in significant repair delays and induction of genomic instability. Upon detection of DNA damage, the corresponding DNA damage response and repair (DDR/R) mechanisms are activated. The inability of cells to process clustered DNA lesions efficiently has a great impact on the normal function and survival of cells. If complex lesions are left unrepaired or misrepaired, they can lead to mutations and if persistent, they may lead to apoptotic cell death. In this in silico study, and through rigorous data mining, we have identified human genes that are activated upon complex DNA damage induction like in the case of ionizing radiation (IR) and beyond the standard DNA repair pathways, and are also involved in cancer pathways, by employing stringent bioinformatics and systems biology methodologies. Given that IR can cause repair resistant lesions within a short DNA segment (a few nm), thereby augmenting the hazardous and toxic effects of radiation, we also investigated the possible implication of the most biologically important of those genes in comorbid non-neoplastic diseases through network integration, as well as their potential for predicting survival in cancer patients. View Full-Text
Keywords: clustered DNA damage; ionizing radiation; cancer; comorbidities; prognosis; systems biology clustered DNA damage; ionizing radiation; cancer; comorbidities; prognosis; systems biology
Show Figures

Graphical abstract

MDPI and ACS Style

Pavlopoulou, A.; Asfa, S.; Gioukakis, E.; Mavragani, I.V.; Nikitaki, Z.; Takan, I.; Pouget, J.-P.; Harrison, L.; Georgakilas, A.G. In Silico Investigation of the Biological Implications of Complex DNA Damage with Emphasis in Cancer Radiotherapy through a Systems Biology Approach. Molecules 2021, 26, 7602. https://doi.org/10.3390/molecules26247602

AMA Style

Pavlopoulou A, Asfa S, Gioukakis E, Mavragani IV, Nikitaki Z, Takan I, Pouget J-P, Harrison L, Georgakilas AG. In Silico Investigation of the Biological Implications of Complex DNA Damage with Emphasis in Cancer Radiotherapy through a Systems Biology Approach. Molecules. 2021; 26(24):7602. https://doi.org/10.3390/molecules26247602

Chicago/Turabian Style

Pavlopoulou, Athanasia, Seyedehsadaf Asfa, Evangelos Gioukakis, Ifigeneia V. Mavragani, Zacharenia Nikitaki, Işıl Takan, Jean-Pierre Pouget, Lynn Harrison, and Alexandros G. Georgakilas. 2021. "In Silico Investigation of the Biological Implications of Complex DNA Damage with Emphasis in Cancer Radiotherapy through a Systems Biology Approach" Molecules 26, no. 24: 7602. https://doi.org/10.3390/molecules26247602

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop