Resveratrol Promotes Angiogenesis in a FoxO1-Dependent Manner in Hind Limb Ischemia in Mice
Abstract
:1. Introduction
2. Results
2.1. RSV Treatment Improves Blood Restoration in Hind Limb Ischemia Mouse Model
2.2. RSV Inhibits FoxO1 Pathway in Ischemic Muscle and HUVECs
2.3. RSV Promotes Angiogenesis in a FoxO1-Dependent Manner
2.4. Differential Metabolites Analysis of Skeletal Muscle Samples
3. Discussion
4. Materials and Methods
4.1. Drug and Animals
4.2. Hind Limb Ischemia Model Establishment and Function Assessment
4.3. Terminal-Deoxynucleotidyl Transferase Mediated Nick End Labeling (TUNEL) Assay
4.4. Immunohistochemical Staining
4.5. Metabolomics Analysis
4.6. Cell Culture and Ischemia Treatments
4.7. Matrigel Tube Formation Assay
4.8. Cell Transfection
4.9. Western Blot
4.10. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Fan, D.; Liu, C.; Guo, Z.; Huang, K.; Peng, M.; Li, N.; Luo, H.; Wang, T.; Cen, Z.; Cai, W.; et al. Resveratrol Promotes Angiogenesis in a FoxO1-Dependent Manner in Hind Limb Ischemia in Mice. Molecules 2021, 26, 7528. https://doi.org/10.3390/molecules26247528
Fan D, Liu C, Guo Z, Huang K, Peng M, Li N, Luo H, Wang T, Cen Z, Cai W, et al. Resveratrol Promotes Angiogenesis in a FoxO1-Dependent Manner in Hind Limb Ischemia in Mice. Molecules. 2021; 26(24):7528. https://doi.org/10.3390/molecules26247528
Chicago/Turabian StyleFan, Dongxiao, Chenshu Liu, Zeling Guo, Kan Huang, Meixiu Peng, Na Li, Hengli Luo, Tengyao Wang, Zhipeng Cen, Weikang Cai, and et al. 2021. "Resveratrol Promotes Angiogenesis in a FoxO1-Dependent Manner in Hind Limb Ischemia in Mice" Molecules 26, no. 24: 7528. https://doi.org/10.3390/molecules26247528