Next Article in Journal
Two New Triterpenes from Basidiomata of the Medicinal and Edible Mushroom, Laetiporus sulphureus
Next Article in Special Issue
Assessment of Anti-Inflammatory and Antimicrobial Potential of Ethanolic Extract of Woodfordia fruticosa Flowers: GC-MS Analysis
Previous Article in Journal
A Comprehensive Analysis of the Metal–Nitrile Bonding in an Organo-Diiron System
Previous Article in Special Issue
Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-κB/IRF3 Signaling Pathways
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 26
Issue 23
10.3390/molecules26237089
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Ginsenoside Rb1 Mitigates Escherichia coli Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway

by
Aftab Shaukat
1,
Irfan Shaukat
2,
Shahid Ali Rajput
3,
Rizwan Shukat
4,
Sana Hanif
5,
Imran Shaukat
6,
Xinxin Zhang
1,
Chao Chen
1,
Xuyang Sun
1,
Tingzhu Ye
1,
Kaifeng Niu
1,
Zhiqiu Yao
1,
Shadab Shaukat
7,
Muhammad Safdar
1,
Mohamed Abdelrahman
1,8,
Umair Riaz
1,
Junwei Zhao
1,
Xiaoying Gu
1 and
Liguo Yang
1,*
1
National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, China
2
Faculty of Medicine, University of Lorraine, 54052 Nancy, France
3
Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan 66000, Pakistan
4
Faculty of Food, Nutrition & Home Sciences, University of Agriculture, Faisalabad 38000, Pakistan
5
Hubei Key Laboratory of Theory and Application of Advanced Materials Mechanics, Wuhan University of Technology, Wuhan 430070, China
6
Department of Physics, University of Agriculture, Faisalabad 38000, Pakistan
7
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, Pakistan
8
Animal Production Department, Faculty of Agriculture, Assuit University, Asyut 71515, Egypt
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(23), 7089; https://doi.org/10.3390/molecules26237089
Submission received: 19 October 2021 / Revised: 16 November 2021 / Accepted: 16 November 2021 / Published: 23 November 2021
(This article belongs to the Special Issue Plant Extracts: Biological and Pharmacological Activity—the Second Edition)
Browse Figures
Versions Notes

Abstract

Endometritis is the inflammatory response of the endometrial lining of the uterus and is associated with low conception rates, early embryonic mortality, and prolonged inter-calving intervals, and thus poses huge economic losses to the dairy industry worldwide. Ginsenoside Rb1 (GnRb1) is a natural compound obtained from the roots of Panax ginseng, having several pharmacological and biological properties. However, the anti-inflammatory properties of GnRb1 in lipopolysaccharide (LPS)-challenged endometritis through the TLR4-mediated NF-κB signaling pathway has not yet been researched. This study was planned to evaluate the mechanisms of how GnRb1 rescues LPS-induced endometritis. In the present research, histopathological findings revealed that GnRb1 ameliorated LPS-triggered uterine injury. The ELISA and RT-qPCR assay findings indicated that GnRb1 suppressed the expression level of pro-inflammatory molecules (TNF-α, IL-1β and IL-6) and boosted the level of anti-inflammatory (IL-10) cytokine. Furthermore, the molecular study suggested that GnRb1 attenuated TLR4-mediated NF-κB signaling. The results demonstrated the therapeutic efficacy of GnRb1 in the mouse model of LPS-triggered endometritis via the inhibition of the TLR4-associated NF-κB pathway. Taken together, this study provides a baseline for the protective effect of GnRb1 to treat endometritis in both humans and animals.
Keywords: endometritis; lipopolysaccharide; Ginsenoside Rb1; TLR4; NF-κB pathway endometritis; lipopolysaccharide; Ginsenoside Rb1; TLR4; NF-κB pathway

Share and Cite

MDPI and ACS Style

Shaukat, A.; Shaukat, I.; Rajput, S.A.; Shukat, R.; Hanif, S.; Shaukat, I.; Zhang, X.; Chen, C.; Sun, X.; Ye, T.; et al. Ginsenoside Rb1 Mitigates Escherichia coli Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway. Molecules 2021, 26, 7089. https://doi.org/10.3390/molecules26237089

AMA Style

Shaukat A, Shaukat I, Rajput SA, Shukat R, Hanif S, Shaukat I, Zhang X, Chen C, Sun X, Ye T, et al. Ginsenoside Rb1 Mitigates Escherichia coli Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway. Molecules. 2021; 26(23):7089. https://doi.org/10.3390/molecules26237089

Chicago/Turabian Style

Shaukat, Aftab, Irfan Shaukat, Shahid Ali Rajput, Rizwan Shukat, Sana Hanif, Imran Shaukat, Xinxin Zhang, Chao Chen, Xuyang Sun, Tingzhu Ye, and et al. 2021. "Ginsenoside Rb1 Mitigates Escherichia coli Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway" Molecules 26, no. 23: 7089. https://doi.org/10.3390/molecules26237089

APA Style

Shaukat, A., Shaukat, I., Rajput, S. A., Shukat, R., Hanif, S., Shaukat, I., Zhang, X., Chen, C., Sun, X., Ye, T., Niu, K., Yao, Z., Shaukat, S., Safdar, M., Abdelrahman, M., Riaz, U., Zhao, J., Gu, X., & Yang, L. (2021). Ginsenoside Rb1 Mitigates Escherichia coli Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway. Molecules, 26(23), 7089. https://doi.org/10.3390/molecules26237089

Article Metrics

Back to TopTop