Next Article in Journal
A Review of the Recent Developments in the Bioproduction of Polylactic Acid and Its Precursors Optically Pure Lactic Acids
Previous Article in Journal
Chalcogen Bond Involving Zinc(II)/Cadmium(II) Carbonate and Its Enhancement by Spodium Bond
Article

Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards

1
Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK
2
National Research Centre, Chemistry of Flavour and Aroma Department, Dokki, Cairo 12622, Egypt
*
Author to whom correspondence should be addressed.
Academic Editor: Yannis Dotsikas
Molecules 2021, 26(21), 6444; https://doi.org/10.3390/molecules26216444
Received: 15 September 2021 / Revised: 13 October 2021 / Accepted: 22 October 2021 / Published: 26 October 2021
(This article belongs to the Section Cross-Field Chemistry)
The gut microbiota is critical to the maintenance of physiological homeostasis and as such is implicated in a range of diseases such as colon cancer, ulcerative colitis, diabetes, cardiovascular diseases, and neurodegenerative diseases. Short chain fatty acids (SCFAs) are key metabolites produced by the gut microbiota from the fermentation of dietary fibre. Here we present a novel, sensitive, and direct LC-MS/MS technique using isotopically labelled internal standards without derivatisation for the analysis of SCFAs in different biological matrices. The technique has significant advantages over the current widely used techniques based on sample derivatization and GC-MS analysis, including fast and simple sample preparation and short LC runtime (10 min). The technique is specific and sensitive for the quantification of acetate, butyrate, isobutyrate, isovalerate, lactate, propionate and valerate. The limits of detection were all 0.001 mM except for acetate which was 0.003 mM. The calibration curves for all the analytes were linear with correlation coefficients r2 > 0.998. The intra- and inter-day precisions in three levels of known concentrations were <12% and <20%, respectively. The quantification accuracy ranged from 92% to 120%. The technique reported here offers a valuable analytical tool for use in studies of SCFA production in the gut and their distribution to host tissues. View Full-Text
Keywords: gut microbiota; kidney; diabetes; neurodegenerative; cardiovascular; plasma; milk; lactate; butyrate; acetate; propionate gut microbiota; kidney; diabetes; neurodegenerative; cardiovascular; plasma; milk; lactate; butyrate; acetate; propionate
Show Figures

Figure 1

MDPI and ACS Style

Saha, S.; Day-Walsh, P.; Shehata, E.; Kroon, P.A. Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards. Molecules 2021, 26, 6444. https://doi.org/10.3390/molecules26216444

AMA Style

Saha S, Day-Walsh P, Shehata E, Kroon PA. Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards. Molecules. 2021; 26(21):6444. https://doi.org/10.3390/molecules26216444

Chicago/Turabian Style

Saha, Shikha, Priscilla Day-Walsh, Emad Shehata, and Paul A. Kroon. 2021. "Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards" Molecules 26, no. 21: 6444. https://doi.org/10.3390/molecules26216444

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop