Next Article in Journal
In Silico and In Vivo Evaluation of SARS-CoV-2 Predicted Epitopes-Based Candidate Vaccine
Previous Article in Journal
Identification of Antiprotozoal Compounds from Buxus sempervirens L. by PLS-Prediction
Article

In Vivo Antimalarial Activity of Leaf Extracts and a Major Compound Isolated from Ranunculus multifidus Forsk

1
Department of Pharmacy, College of Medicine and Health Sciences, Arba Minch University, Arba Minch P.O. Box 21, Ethiopia
2
Department of Pharmaceutical Chemistry, Faculty of Natural Sciences, Martin-Luther-Universitaet Halle-Wittenberg, 3, 06120 Halle (Saale), Germany
3
Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia
*
Authors to whom correspondence should be addressed.
Academic Editor: Valeria Patricia Sülsen
Molecules 2021, 26(20), 6179; https://doi.org/10.3390/molecules26206179
Received: 20 August 2021 / Revised: 5 October 2021 / Accepted: 8 October 2021 / Published: 13 October 2021
(This article belongs to the Section Natural Products Chemistry)
The leaves of Ranunculus multifidus Forsk. are traditionally used for the treatment of malaria in several African countries. In the present study, 80% methanol (RM-M) and hydrodistilled (RM-H) extracts of fresh leaves from R. multifidus and its major constituent anemonin were tested for their in vivo antimalarial activity against Plasmodium berghei in mice. Anemonin was also tested for its in vitro antimycobacterial activity against Mycobacterium smegmatis and M. abscessus in a microbroth dilution assay, and bacterial growth was analyzed by OD measurement. The isolation of anemonin from RM-H was carried out using preparative thin layer chromatography (PTLC). The chemical structures of anemonin and its hydrolysis product were elucidated using spectroscopic methods (HR–MS; 1D and 2D-NMR). Results of the study revealed that both RM-M and RM-H were active against P. berghei in mice, although the latter demonstrated superior activity (p < 0.001), as compared to the former. At a dose of 35.00 mg/kg/day, RM-H demonstrated a chemosuppression value of 70% in a 4-day suppressive test. In a 4-day suppressive, Rane’s and prophylactic antimalarial tests, anemonin showed median effective doses (ED50s) of 2.17, 2.78 and 2.70 μM, respectively. However, anemonin did not inhibit the growth of M. smegmatis and M. abscessus. View Full-Text
Keywords: Ranunculus multifidus; 4-day suppressive test; Rane’s test; prophylactic test; anemonin Ranunculus multifidus; 4-day suppressive test; Rane’s test; prophylactic test; anemonin
Show Figures

Figure 1

MDPI and ACS Style

Sirak, B.; Mann, L.; Richter, A.; Asres, K.; Imming, P. In Vivo Antimalarial Activity of Leaf Extracts and a Major Compound Isolated from Ranunculus multifidus Forsk. Molecules 2021, 26, 6179. https://doi.org/10.3390/molecules26206179

AMA Style

Sirak B, Mann L, Richter A, Asres K, Imming P. In Vivo Antimalarial Activity of Leaf Extracts and a Major Compound Isolated from Ranunculus multifidus Forsk. Molecules. 2021; 26(20):6179. https://doi.org/10.3390/molecules26206179

Chicago/Turabian Style

Sirak, Betelhem, Lea Mann, Adrian Richter, Kaleab Asres, and Peter Imming. 2021. "In Vivo Antimalarial Activity of Leaf Extracts and a Major Compound Isolated from Ranunculus multifidus Forsk" Molecules 26, no. 20: 6179. https://doi.org/10.3390/molecules26206179

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop