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Article

Effect of Hydrolyzable Tannins on Glucose-Transporter Expression and Their Bioavailability in Pig Small-Intestinal 3D Cell Model

1
Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, Slovenia
2
Veterinary Faculty, Institute of Preclinical Sciences, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
3
Center for Human Molecular Genetics and Pharmacogenomics, Faculty of Medicine, University of Maribor, Taborska 8, 2000 Maribor, Slovenia
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Department of Chemistry, Faculty of Medicine, University of Maribor, Taborska 8, 2000 Maribor, Slovenia
5
Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(2), 345; https://doi.org/10.3390/molecules26020345
Received: 2 November 2020 / Revised: 27 December 2020 / Accepted: 6 January 2021 / Published: 11 January 2021
(This article belongs to the Special Issue Tannin Analysis, Chemistry, and Functions)
Intestinal transepithelial transport of glucose is mediated by glucose transporters, and affects postprandial blood-glucose levels. This study investigates the effect of wood extracts rich in hydrolyzable tannins (HTs) that originated from sweet chestnut (Castanea sativa Mill.) and oak (Quercus petraea) on the expression of glucose transporter genes and the uptake of glucose and HT constituents in a 3D porcine-small-intestine epithelial-cell model. The viability of epithelial cells CLAB and PSI exposed to different HTs was determined using alamarBlue®. qPCR was used to analyze the gene expression of SGLT1, GLUT2, GLUT4, and POLR2A. Glucose uptake was confirmed by assay, and LC–MS/ MS was used for the analysis of HT bioavailability. HTs at 37 µg/mL were found to adversely affect cell viability and downregulate POLR2A expression. HT from wood extract Tanex at concentrations of 4 µg/mL upregulated the expression of GLUT2, as well as glucose uptake at 1 µg/mL. The time-dependent passage of gallic acid through enterocytes was influenced by all wood extracts compared to gallic acid itself as a control. These results suggest that HTs could modulate glucose uptake and gallic acid passage in the 3D cell model. View Full-Text
Keywords: hydrolyzable tannin; glucose; glucose transporters; porcine epithelial cell line; tannin bioavailability; glucose transport hydrolyzable tannin; glucose; glucose transporters; porcine epithelial cell line; tannin bioavailability; glucose transport
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MDPI and ACS Style

Brus, M.; Frangež, R.; Gorenjak, M.; Kotnik, P.; Knez, Ž.; Škorjanc, D. Effect of Hydrolyzable Tannins on Glucose-Transporter Expression and Their Bioavailability in Pig Small-Intestinal 3D Cell Model. Molecules 2021, 26, 345. https://doi.org/10.3390/molecules26020345

AMA Style

Brus M, Frangež R, Gorenjak M, Kotnik P, Knez Ž, Škorjanc D. Effect of Hydrolyzable Tannins on Glucose-Transporter Expression and Their Bioavailability in Pig Small-Intestinal 3D Cell Model. Molecules. 2021; 26(2):345. https://doi.org/10.3390/molecules26020345

Chicago/Turabian Style

Brus, Maksimiljan, Robert Frangež, Mario Gorenjak, Petra Kotnik, Željko Knez, and Dejan Škorjanc. 2021. "Effect of Hydrolyzable Tannins on Glucose-Transporter Expression and Their Bioavailability in Pig Small-Intestinal 3D Cell Model" Molecules 26, no. 2: 345. https://doi.org/10.3390/molecules26020345

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