Next Article in Journal
Design, Synthesis, Antibacterial, and Antitumor Activity of Linear Polyisocyanide Quaternary Ammonium Salts with Different Structures and Chain Lengths
Next Article in Special Issue
Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE
Previous Article in Journal
Simultaneous Determination of Tetracyclines and Fluoroquinolones in Poultry Eggs by UPLC Integrated with Dual-Channel-Fluorescence Detection Method
Previous Article in Special Issue
Polypeptide N-acetylgalactosaminyltransferase-Associated Phenotypes in Mammals
 
 
Correction published on 31 May 2022, see Molecules 2022, 27(11), 3534.
Article

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Hideyuki Takeuchi
Molecules 2021, 26(18), 5685; https://doi.org/10.3390/molecules26185685
Received: 29 July 2021 / Revised: 14 September 2021 / Accepted: 16 September 2021 / Published: 19 September 2021 / Corrected: 31 May 2022
(This article belongs to the Special Issue New Insights into Protein Glycosylation)
DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation. View Full-Text
Keywords: C-mannosylation; glycobiology; DPY19L3; myotube; myogenic differentiation C-mannosylation; glycobiology; DPY19L3; myotube; myogenic differentiation
Show Figures

Figure 1

MDPI and ACS Style

Mori, K.; Sun, H.; Miura, K.; Simizu, S. Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts. Molecules 2021, 26, 5685. https://doi.org/10.3390/molecules26185685

AMA Style

Mori K, Sun H, Miura K, Simizu S. Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts. Molecules. 2021; 26(18):5685. https://doi.org/10.3390/molecules26185685

Chicago/Turabian Style

Mori, Kento, Hongkai Sun, Kazuki Miura, and Siro Simizu. 2021. "Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts" Molecules 26, no. 18: 5685. https://doi.org/10.3390/molecules26185685

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop