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Article

Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms

1
UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France
2
CHRU Tours, Medical Biology Center, 37000 Tours, France
3
ALLICE, Phenotyping Station, 37380 Nouzilly, France
*
Author to whom correspondence should be addressed.
Academic Editor: Giorgio Marrubini
Molecules 2021, 26(14), 4111; https://doi.org/10.3390/molecules26144111
Received: 15 June 2021 / Revised: 28 June 2021 / Accepted: 28 June 2021 / Published: 6 July 2021
(This article belongs to the Special Issue Method Development of Sampling Preparation Techniques)
Currently, most clinical studies in metabolomics only consider a single type of sample such as urine, plasma, or feces and use a single analytical platform, either NMR or MS. Although some studies have already investigated metabolomics data from multiple fluids, the information is limited to a unique analytical platform. On the other hand, clinical studies investigating the human metabolome that combine multi-analytical platforms have focused on a single biofluid. Combining data from multiple sample types for one patient using a multimodal analytical approach (NMR and MS) should extend the metabolome coverage. Pre-analytical and analytical phases are time consuming. These steps need to be improved in order to move into clinical studies that deal with a large number of patient samples. Our study describes a standard operating procedure for biological specimens (urine, blood, saliva, and feces) using multiple platforms (1H-NMR, RP-UHPLC-MS, and HILIC-UHPLC-MS). Each sample type follows a unique sample preparation procedure for analysis on a multi-platform basis. Our method was evaluated for its robustness and was able to generate a representative metabolic map. View Full-Text
Keywords: biological fluids; extraction protocol; UHPLC-HRMS; 1H-NMR; metabolomic card biological fluids; extraction protocol; UHPLC-HRMS; 1H-NMR; metabolomic card
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MDPI and ACS Style

Martias, C.; Baroukh, N.; Mavel, S.; Blasco, H.; Lefèvre, A.; Roch, L.; Montigny, F.; Gatien, J.; Schibler, L.; Dufour-Rainfray, D.; Nadal-Desbarats, L.; Emond, P. Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms. Molecules 2021, 26, 4111. https://doi.org/10.3390/molecules26144111

AMA Style

Martias C, Baroukh N, Mavel S, Blasco H, Lefèvre A, Roch L, Montigny F, Gatien J, Schibler L, Dufour-Rainfray D, Nadal-Desbarats L, Emond P. Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms. Molecules. 2021; 26(14):4111. https://doi.org/10.3390/molecules26144111

Chicago/Turabian Style

Martias, Cécile, Nadine Baroukh, Sylvie Mavel, Hélène Blasco, Antoine Lefèvre, Léa Roch, Frédéric Montigny, Julie Gatien, Laurent Schibler, Diane Dufour-Rainfray, Lydie Nadal-Desbarats, and Patrick Emond. 2021. "Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms" Molecules 26, no. 14: 4111. https://doi.org/10.3390/molecules26144111

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