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Article

Production of Siderophores by an Apple Root-Associated Streptomyces ciscaucasicus Strain GS2 Using Chemical and Biological OSMAC Approaches

1
Center for Mass Spectrometry (CMS), Faculty of Chemistry and Chemical Biology, Technische Universität Dortmund, Otto-Hahn-Str. 6, 44227 Dortmund, Germany
2
Institute of Horticultural Production Systems, Section Phytomedicine, Leibniz Universität Hannover, Herrenhäuser Str. 2, 30419 Hannover, Germany
3
Institute of Horticultural Production Systems, Section Woody Plant and Propagation Physiology, Leibniz Universität Hannover, Herrenhäuser Str. 2, 30419 Hannover, Germany
*
Author to whom correspondence should be addressed.
Present address: Environmental Analysis, Currenta GmbH & Co. OHG, Chempark Blg Q18, 51368 Leverkusen, Germany.
Present address: Instrumental Analytical Chemistry (IAC) and Centre for Water and Environmental Research (ZWU), University of Duisburg-Essen, Universitätsstrasse 5, 45141 Essen, Germany.
Academic Editor: Gilles Comte
Molecules 2021, 26(12), 3517; https://doi.org/10.3390/molecules26123517
Received: 8 May 2021 / Revised: 1 June 2021 / Accepted: 3 June 2021 / Published: 9 June 2021
(This article belongs to the Special Issue Natural Products from Plant-Associated Microorganisms)
Apple Replant Disease (ARD) is a significant problem in apple orchards that causes root tissue damage, stunted plant growth, and decline in fruit quality, size, and overall yield. Dysbiosis of apple root-associated microbiome and selective richness of Streptomyces species in the rhizosphere typically concurs root impairment associated with ARD. However, possible roles of Streptomyces secondary metabolites within these observations remain unstudied. Therefore, we employed the One Strain Many Compounds (OSMAC) approach coupled to high-performance liquid chromatography-high-resolution tandem mass spectrometry (HPLC-HRMSn) to evaluate the chemical ecology of an apple root-associated Streptomycesciscaucasicus strain GS2, temporally over 14 days. The chemical OSMAC approach comprised cultivation media alterations using six different media compositions, which led to the biosynthesis of the iron-chelated siderophores, ferrioxamines. The biological OSMAC approach was concomitantly applied by dual-culture cultivation for microorganismal interactions with an endophytic Streptomyces pulveraceus strain ES16 and the pathogen Cylindrocarpon olidum. This led to the modulation of ferrioxamines produced and further triggered biosynthesis of the unchelated siderophores, desferrioxamines. The structures of the compounds were elucidated using HRMSn and by comparison with the literature. We evaluated the dynamics of siderophore production under the combined influence of chemical and biological OSMAC triggers, temporally over 3, 7, and 14 days, to discern the strain’s siderophore-mediated chemical ecology. We discuss our results based on the plausible chemical implications of S. ciscaucasicus strain GS2 in the rhizosphere. View Full-Text
Keywords: Apple Replant Disease (ARD); HPLC-HRMS; HPLC-HRMS/MS; One Strain Many Compounds (OSMAC); siderophores; Streptomyces ciscaucasicus Apple Replant Disease (ARD); HPLC-HRMS; HPLC-HRMS/MS; One Strain Many Compounds (OSMAC); siderophores; Streptomyces ciscaucasicus
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MDPI and ACS Style

Armin, R.; Zühlke, S.; Grunewaldt-Stöcker, G.; Mahnkopp-Dirks, F.; Kusari, S. Production of Siderophores by an Apple Root-Associated Streptomyces ciscaucasicus Strain GS2 Using Chemical and Biological OSMAC Approaches. Molecules 2021, 26, 3517. https://doi.org/10.3390/molecules26123517

AMA Style

Armin R, Zühlke S, Grunewaldt-Stöcker G, Mahnkopp-Dirks F, Kusari S. Production of Siderophores by an Apple Root-Associated Streptomyces ciscaucasicus Strain GS2 Using Chemical and Biological OSMAC Approaches. Molecules. 2021; 26(12):3517. https://doi.org/10.3390/molecules26123517

Chicago/Turabian Style

Armin, Reyhaneh, Sebastian Zühlke, Gisela Grunewaldt-Stöcker, Felix Mahnkopp-Dirks, and Souvik Kusari. 2021. "Production of Siderophores by an Apple Root-Associated Streptomyces ciscaucasicus Strain GS2 Using Chemical and Biological OSMAC Approaches" Molecules 26, no. 12: 3517. https://doi.org/10.3390/molecules26123517

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