Gossypitrin, A Naturally Occurring Flavonoid, Attenuates Iron-Induced Neuronal and Mitochondrial Damage

Round 1

Reviewer 1 Report

Thanks for the opportunity to review this manuscript. The subject is really important because of the growing number of patients with neurological diseases, which carries a large socio-economic problems. A study identifying a new, natural substances to support the treatment of these diseases which are extremely relevant. Nevertheless, in my opinion, a few issues should be clarified before the publication of the article.

1. I have the biggest reservations about the discussion. In my opinion, this is a more detailed description of the obtained results than a methodical discussion of the obtained results with other studies. Moreover, for me, the work would be more understandable if it included a description of the signaling pathways explaining the mechanism of action of gossypitrin.
2. The language is incomprehensible in some places, linguistic correction is required
3. The quality of the figures and signatures is not very satisfactory

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

The manuscript entitled “Gossypitrin, a naturally occurring flavonoid, attenuates iron-induced neuronal and mitochondrial damage” by Bécquer-Viart et al. reports the Gossypitrin ability to protect from neuronal diseases associated with iron-induced oxidative stress and mitochondrial damage.

In the introduction the topics is properly illustrated. The authors reported a detailed biological section supported by a good rational and all experiments are properly described.

The manuscript is well written, and suitable for publication in Molecules after revision of few points:

Introduction:

“The presence of a planar six-member cyclic system with electron delocalization, a catechol moiety, and three more aromatic hydroxyl groups (two of them adjacent to a carbonyl group), strongly suggest that this molecule could act as an iron-chelating agent”

My suggestion is to focus on this point supporting it with the literature. Are there any references in literature about the iron-chelating properties of structures that are similar to Gos?

Line 49: There is a missing bracket

Results:

The presence of letters on the histograms is confusing. My suggestion is to insert a legenda in the caption of each figures and/or to remove the letters where there are not necessary.

Figure 6B: the comparison of IR spectra of Gos and Gos-Fe is described. It could be interesting to compare also the ¹HNMR of these two samples.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Thank you for responding to my comments and for revising the manuscript.