Next Article in Journal
Lactoferrin: A Glycoprotein Involved in Immunomodulation, Anticancer, and Antimicrobial Processes
Previous Article in Journal
Phytochemical Characterization, In Vitro Anti-Inflammatory, Anti-Diabetic, and Cytotoxic Activities of the Edible Aromatic Plant; Pulicaria jaubertii
Article

Binding Characteristics Study of DNA based Aptamers for E. coli O157:H7

by 1,* and 2
1
Department of Bioengineering, Hong Kong University of Science and Technology, Kowloon, Hong Kong
2
Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Kowloon, Hong Kong
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(1), 204; https://doi.org/10.3390/molecules26010204
Received: 20 November 2020 / Revised: 21 December 2020 / Accepted: 29 December 2020 / Published: 3 January 2021
E. coli O157:H7 is a pathogenic bacterium producing verotoxins that could lead to serious complications such as hemolytic uremia syndrome. Fast detection of such pathogens is important. For rapid detection, aptamers are quickly gaining traction as alternative biorecognition molecules besides conventional antibodies. Several DNA aptamers have been selected for E. coli O157:H7. Nonetheless, there has not been a comparative study of the binding characteristics of these aptamers. In this work, we present a comprehensive analysis of binding characteristics including binding affinity (Kd) and binding capacity (Bmax) of DNA-based aptamers for E. coli O157:H7 using qPCR. Our results show that aptamer E18R has the highest binding capacity to E. coli 157:H7 and the highest specificity over non-pathogenic E. coli strains K12 and DH5α. Our study also finds that the common biotin-tag modification at 5′ end typically changes the binding capacity significantly. For most of the selected aptamers, the binding capacity after a biotin-tag modification decreases. There exists a discrepancy in the binding capability between the selected aptamer and the aptamer used for detection. Our study also shows that a lower concentration of Mg2+ ions in the binding buffer leads to a decrease in the binding capacity of E17F and E18R, while it does not affect the binding capacity of S1 and EcoR1. View Full-Text
Keywords: DNA aptamers; E. coli O157:H7 detection; aptamer binding characteristics; biotin modification; ionic buffer strength DNA aptamers; E. coli O157:H7 detection; aptamer binding characteristics; biotin modification; ionic buffer strength
Show Figures

Figure 1

MDPI and ACS Style

Siddiqui, S.; Yuan, J. Binding Characteristics Study of DNA based Aptamers for E. coli O157:H7. Molecules 2021, 26, 204. https://doi.org/10.3390/molecules26010204

AMA Style

Siddiqui S, Yuan J. Binding Characteristics Study of DNA based Aptamers for E. coli O157:H7. Molecules. 2021; 26(1):204. https://doi.org/10.3390/molecules26010204

Chicago/Turabian Style

Siddiqui, Saika, and Jie Yuan. 2021. "Binding Characteristics Study of DNA based Aptamers for E. coli O157:H7" Molecules 26, no. 1: 204. https://doi.org/10.3390/molecules26010204

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop