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Open AccessArticle

A Two-Step Ferric Chloride and Dilute Alkaline Pretreatment for Enhancing Enzymatic Hydrolysis and Fermentable Sugar Recovery from Miscanthus sinensis

College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
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These authors have contributed equally to this work.
Molecules 2020, 25(8), 1843; https://doi.org/10.3390/molecules25081843
Received: 12 February 2020 / Revised: 12 April 2020 / Accepted: 15 April 2020 / Published: 16 April 2020
A two-step process was proposed to enhance enzymatic hydrolysis of Miscanthus sinensis based on a comparative study of acid/alkaline pretreatments. Ferric chloride pretreatment (FP) effectively removed hemicellulose and recovered soluble sugars, but the enzymatic hydrolysis was not efficient. Dilute alkaline pretreatment (ALP) resulted in much better delignification and stronger morphological changes of the sample, making it more accessible to enzymes. While ALP obtained the highest sugar yield during enzymatic hydrolysis, the soluble sugar recovery from the pretreatment stage was still limited. Furthermore, a two-step ferric chloride and dilute alkaline pretreatment (F-ALP) has been successfully developed by effectively recovering soluble sugars in the first FP step and further removing lignin of the FP sample in the second ALP step to improve its enzymatic hydrolysis. As a result, the two-step process yielded the highest total sugar recovery (418.8 mg/g raw stalk) through the whole process. View Full-Text
Keywords: two-step pretreatment; Miscanthus sinensis; enzymatic hydrolysis; sugar recovery two-step pretreatment; Miscanthus sinensis; enzymatic hydrolysis; sugar recovery
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Li, L.; Ye, P.; Chen, M.; Tang, S.; Luo, Y.; Gao, Y.; Yan, Q.; Cheng, X. A Two-Step Ferric Chloride and Dilute Alkaline Pretreatment for Enhancing Enzymatic Hydrolysis and Fermentable Sugar Recovery from Miscanthus sinensis. Molecules 2020, 25, 1843.

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